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The Reduced Level of Inorganic Polyphosphate Mobilizes Antioxidant and Manganese-Resistance Systems in Saccharomyces cerevisiae

1
Skryabin Institute of Biochemistry and Physiology of Microorganisms, FRC Pushchino Center for Biological Research of the Russian Academy of Sciences, pr. Nauki 5, Pushchino 142290, Russia
2
Institute for Information Transmission Problems, Russian Academy of Sciences, Bolshoy Karetny per. 19 bld .1, Moscow 127051, Russia
3
Institute of Protein Research, Russian Academy of Sciences, Institutskaya 4, Pushchino 142290, Russia
4
Institute of Physicochemical and Biological Problems of Soil Science, FRC Pushchino Center for Biological Research of the Russian Academy of Sciences, pr. Nauki 2, Pushchino 142290, Russia
5
Vavilov Institute of General Genetics, Russian Academy of Sciences, Gubkina 3, Moscow GSP-1 119991, Russia
6
Engelhardt Institute of Molecular Biology, Russian Academy of Sciences, Vavilova 32, Moscow GSP-1 119991, Russia
7
Institute of Mathematical Problems of Biology RAS—the Branch of Keldysh Institute of Applied Mathematics of Russian Academy of Sciences, Vitkevicha 1, Pushchino 142290, Russia
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(5), 461; https://doi.org/10.3390/cells8050461
Received: 16 April 2019 / Revised: 13 May 2019 / Accepted: 15 May 2019 / Published: 15 May 2019
(This article belongs to the Special Issue Molecular and Cellular Mechanisms of Stress Responses)
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Abstract

Inorganic polyphosphate (polyP) is crucial for adaptive reactions and stress response in microorganisms. A convenient model to study the role of polyP in yeast is the Saccharomyces cerevisiae strain CRN/PPN1 that overexpresses polyphosphatase Ppn1 with stably decreased polyphosphate level. In this study, we combined the whole-transcriptome sequencing, fluorescence microscopy, and polyP quantification to characterize the CRN/PPN1 response to manganese and oxidative stresses. CRN/PPN1 exhibits enhanced resistance to manganese and peroxide due to its pre-adaptive state observed in normal conditions. The pre-adaptive state is characterized by up-regulated genes involved in response to an external stimulus, plasma membrane organization, and oxidation/reduction. The transcriptome-wide data allowed the identification of particular genes crucial for overcoming the manganese excess. The key gene responsible for manganese resistance is PHO84 encoding a low-affinity manganese transporter: Strong PHO84 down-regulation in CRN/PPN1 increases manganese resistance by reduced manganese uptake. On the contrary, PHM7, the top up-regulated gene in CRN/PPN1, is also strongly up-regulated in the manganese-adapted parent strain. Phm7 is an unannotated protein, but manganese adaptation is significantly impaired in Δphm7, thus suggesting its essential function in manganese or phosphate transport. View Full-Text
Keywords: polyphosphate; PPN1; PHM7; PHO84; manganese adaptation; oxidative stress; Saccharomyces cerevisiae polyphosphate; PPN1; PHM7; PHO84; manganese adaptation; oxidative stress; Saccharomyces cerevisiae
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Trilisenko, L.; Zvonarev, A.; Valiakhmetov, A.; Penin, A.A.; Eliseeva, I.A.; Ostroumov, V.; Kulakovskiy, I.V.; Kulakovskaya, T. The Reduced Level of Inorganic Polyphosphate Mobilizes Antioxidant and Manganese-Resistance Systems in Saccharomyces cerevisiae. Cells 2019, 8, 461.

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