The Reduced Level of Inorganic Polyphosphate Mobilizes Antioxidant and Manganese-Resistance Systems in Saccharomyces cerevisiae
AbstractInorganic polyphosphate (polyP) is crucial for adaptive reactions and stress response in microorganisms. A convenient model to study the role of polyP in yeast is the Saccharomyces cerevisiae strain CRN/PPN1 that overexpresses polyphosphatase Ppn1 with stably decreased polyphosphate level. In this study, we combined the whole-transcriptome sequencing, fluorescence microscopy, and polyP quantification to characterize the CRN/PPN1 response to manganese and oxidative stresses. CRN/PPN1 exhibits enhanced resistance to manganese and peroxide due to its pre-adaptive state observed in normal conditions. The pre-adaptive state is characterized by up-regulated genes involved in response to an external stimulus, plasma membrane organization, and oxidation/reduction. The transcriptome-wide data allowed the identification of particular genes crucial for overcoming the manganese excess. The key gene responsible for manganese resistance is PHO84 encoding a low-affinity manganese transporter: Strong PHO84 down-regulation in CRN/PPN1 increases manganese resistance by reduced manganese uptake. On the contrary, PHM7, the top up-regulated gene in CRN/PPN1, is also strongly up-regulated in the manganese-adapted parent strain. Phm7 is an unannotated protein, but manganese adaptation is significantly impaired in Δphm7, thus suggesting its essential function in manganese or phosphate transport. View Full-Text
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Trilisenko, L.; Zvonarev, A.; Valiakhmetov, A.; Penin, A.A.; Eliseeva, I.A.; Ostroumov, V.; Kulakovskiy, I.V.; Kulakovskaya, T. The Reduced Level of Inorganic Polyphosphate Mobilizes Antioxidant and Manganese-Resistance Systems in Saccharomyces cerevisiae. Cells 2019, 8, 461.
Trilisenko L, Zvonarev A, Valiakhmetov A, Penin AA, Eliseeva IA, Ostroumov V, Kulakovskiy IV, Kulakovskaya T. The Reduced Level of Inorganic Polyphosphate Mobilizes Antioxidant and Manganese-Resistance Systems in Saccharomyces cerevisiae. Cells. 2019; 8(5):461.Chicago/Turabian Style
Trilisenko, Ludmila; Zvonarev, Anton; Valiakhmetov, Airat; Penin, Alexey A.; Eliseeva, Irina A.; Ostroumov, Vladimir; Kulakovskiy, Ivan V.; Kulakovskaya, Tatiana. 2019. "The Reduced Level of Inorganic Polyphosphate Mobilizes Antioxidant and Manganese-Resistance Systems in Saccharomyces cerevisiae." Cells 8, no. 5: 461.
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