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From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index

1
Laboratory of Immunology and Cell Biotechnology, Immanuel Kant Baltic Federal University, Kaliningrad 236016, Russia
2
Department of Reconstructive and Endoscopic Surgery, Kaliningrad Regional Hospital, Kaliningrad 236016, Russia
3
Center for Mitochondrial Functional Genomics, Institute of Living Systems, Immanuel Kant Baltic Federal University, Kaliningrad 236040, Russia
4
Center for Integrative Genomics, University of Lausanne, 1015 Lausanne, Switzerland
5
Swiss Institute of Bioinformatics, 1015 Lausanne, Switzerland
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(5), 430; https://doi.org/10.3390/cells8050430
Received: 28 February 2019 / Revised: 23 April 2019 / Accepted: 8 May 2019 / Published: 9 May 2019
(This article belongs to the Special Issue Mitochondrial Genetics)
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Abstract

Mitochondrial DNA (mtDNA) encodes core subunits of oxidative phosphorylation complexes and, as a result of intricate regulatory crosstalk between nuclear and mitochondrial genomes, the total number of mtDNA copies fits the requirements of each cell type. Deviations from the physiological number of mtDNA copies are expected to be deleterious and might cause some inherited diseases and normal ageing. We studied 46 obese patients with type 2 diabetes (T2DM) one year after a laparoscopic sleeve gastrectomy (LSG) and Roux-en-Y gastric bypass (RYGB). The results were compared with normal-weight patients without T2DM (control group 1) (body mass index (BMI) = 22.5 ± 3.01 kg/m2) and patients with obesity without T2DM (control group 2) (BMI = 36 ± 3.45 kg/m2). We detected an increase of mtDNA copy number in the cells of the buffy coat obtained from peripheral blood, sampled one year after bariatric surgery. We also found that average mtDNA copy number as well as its dynamics (before and after the surgery) are gender-specific. To the best of our knowledge, this is the first evidence for the restoration of mtDNA copy number in obese patients after LSG and RYGB. View Full-Text
Keywords: mtDNA copies; bariatric surgery; type 2 diabetes; obesity; ddPCR mtDNA copies; bariatric surgery; type 2 diabetes; obesity; ddPCR
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Skuratovskaia, D.; Litvinova, L.; Vulf, M.; Zatolokin, P.; Popadin, K.; Mazunin, I. From Normal to Obesity and Back: The Associations between Mitochondrial DNA Copy Number, Gender, and Body Mass Index. Cells 2019, 8, 430.

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