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Platelet Lysate Inhibits NF-κB Activation and Induces Proliferation and an Alert State in Quiescent Human Umbilical Vein Endothelial Cells Retaining Their Differentiation Capability

1
Department of Experimental Medicine, University of Genova, via Alberti 2, 16132 Genova, Italy
2
Department of Internal Medicine, University of Genova, via Alberti 2, 16132 Genova, Italy
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2019, 8(4), 331; https://doi.org/10.3390/cells8040331
Received: 18 December 2018 / Revised: 2 April 2019 / Accepted: 8 April 2019 / Published: 9 April 2019
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Abstract

Injured blood vessel repair and blood circulation re-establishment are crucial events for tissue repair. We investigated in primary cultures of human umbilical vein endothelial cells (HUVEC), the effects of platelet lysate (PL), a cocktail of factors released by activated platelets following blood vessel disruption and involved in the wound-healing process triggering. PL exerted a protective effect on HUVEC in an inflammatory milieu by inhibiting IL-1α-activated NF-κB pathway and by inducing the secretion of PGE2, a pro-resolving molecule in the wound microenvironment. Moreover, PL enhanced HUVEC proliferation, without affecting their capability of forming tube-like structures on matrigel, and activated resting quiescent cells to re-enter cell cycle. In agreement with these findings, proliferation-related pathways Akt and ERK1/2 were activated. The expression of the cell-cycle activator Cyclin D1 was also enhanced, as well as the expression of the High Mobility Group Box-1 (HMGB1), a protein of the alarmin group involved in tissue homeostasis, repair, and remodeling. These in vitro data suggest a possible in vivo contribution of PL to new vessel formation after a wound by activation of cells resident in vessel walls. Our biochemical study provides a rationale for the clinical use of PL in the treatment of wound healing-related pathologies. View Full-Text
Keywords: endothelial cells; human umbilical vein endothelial cells (HUVEC); platelet lysate (PL); platelet factors; angiogenesis; ERK; AKT; HMGB-1 endothelial cells; human umbilical vein endothelial cells (HUVEC); platelet lysate (PL); platelet factors; angiogenesis; ERK; AKT; HMGB-1
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Romaldini, A.; Ulivi, V.; Nardini, M.; Mastrogiacomo, M.; Cancedda, R.; Descalzi, F. Platelet Lysate Inhibits NF-κB Activation and Induces Proliferation and an Alert State in Quiescent Human Umbilical Vein Endothelial Cells Retaining Their Differentiation Capability. Cells 2019, 8, 331.

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