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Cells 2019, 8(2), 102; https://doi.org/10.3390/cells8020102

Integration of Transcriptome, Proteome, and Metabolome Provides Insights into How Calcium Enhances the Mechanical Strength of Herbaceous Peony Inflorescence Stems

1,2,†
,
1,†
,
1
,
1,2
,
1,2
,
3
and
1,2,*
1
Jiangsu Key Laboratory of Crop Genetics and Physiology, College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China
2
Institute of Flowers and Trees Industry, Yangzhou University-Rugao City, Rugao 226500, China
3
Ottawa Research and Development Centre, Science and Technology Branch, Agriculture and Agri-Food Canada, Ottawa, ON K1A 0A1, Canada
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Received: 14 December 2018 / Revised: 23 January 2019 / Accepted: 28 January 2019 / Published: 30 January 2019
(This article belongs to the Section Cell Signaling and Regulated Cell Death)
Full-Text   |   PDF [8537 KB, uploaded 30 January 2019]   |  

Abstract

Weak stem mechanical strength severely restrains cut flowers quality and stem weakness can be alleviated by calcium (Ca) treatment, but the mechanisms underlying Ca-mediated enhancement of stem mechanical strength remain largely unknown. In this study, we performed a comparative transcriptomic, proteomic, and metabolomic analysis of herbaceous peony (Paeonia lactiflora Pall.) inflorescence stems treated with nanometer Ca carbonate (Nano-CaCO3). In total, 2643 differentially expressed genes (DEGs) and 892 differentially expressed proteins (DEPs) were detected between the Control and nano-CaCO3 treatment. Among the 892 DEPs, 152 were coregulated at both the proteomic and transcriptomic levels, and 24 DEPs related to the secondary cell wall were involved in signal transduction, energy metabolism, carbohydrate metabolism and lignin biosynthesis, most of which were upregulated after nano-CaCO3 treatment during the development of inflorescence stems. Among these four pathways, numerous differentially expressed metabolites (DEMs) related to lignin biosynthesis were identified. Furthermore, structural observations revealed the thickening of the sclerenchyma cell walls, and the main wall constitutive component lignin accumulated significantly in response to nano-CaCO3 treatment, thereby indicating that Ca can enhance the mechanical strength of the inflorescence stems by increasing the lignin accumulation. These results provided insights into how Ca treatment enhances the mechanical strength of inflorescence stems in P. lactiflora. View Full-Text
Keywords: inflorescence stem; mechanical strength; lignin; cut flower quality; calcium inflorescence stem; mechanical strength; lignin; cut flower quality; calcium
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Zhao, D.; Tang, Y.; Xia, X.; Sun, J.; Meng, J.; Shang, J.; Tao, J. Integration of Transcriptome, Proteome, and Metabolome Provides Insights into How Calcium Enhances the Mechanical Strength of Herbaceous Peony Inflorescence Stems. Cells 2019, 8, 102.

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