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When Unity Is Strength: The Strategies Used by Chlamydomonas to Survive Environmental Stresses
Open AccessArticle

Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations

1
Theoretical Physical Chemistry, MolSys Research Unit, University of Liège, 4000 Liège, Belgium
2
Genetics and Physiology of Microalgae, InBios/Phytosystems Research Unit, University of Liège, 4000 Liège, Belgium
3
Mass Spectrometry Laboratory, MolSys Research Unit, University of Liège, 4000 Liège, Belgium
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The Fritz Haber Research Center for Molecular Dynamics, Institute of Chemistry, Hebrew University of Jerusalem, Jerusalem 91904, Israel
5
Department of Molecular and Medical Pharmacology, David Geffen School of Medicine, University of California Los Angeles, Los Angeles, CA 90095, USA
*
Authors to whom correspondence should be addressed.
Cells 2019, 8(11), 1367; https://doi.org/10.3390/cells8111367
Received: 14 September 2019 / Revised: 23 October 2019 / Accepted: 30 October 2019 / Published: 31 October 2019
(This article belongs to the Special Issue Chlamydomonas Cell Biology)
Acetate can be efficiently metabolized by the green microalga Chlamydomonas reinhardtii. The regular concentration is 17 mM, although higher concentrations are reported to increase starch and fatty acid content. To understand the responses to higher acetate concentrations, Chlamydomonas cells were cultivated in batch mode in the light at 17, 31, 44, and 57 mM acetate. Metabolic analyses show that cells grown at 57 mM acetate possess increased contents of all components analyzed (starch, chlorophylls, fatty acids, and proteins), with a three-fold increased volumetric biomass yield compared to cells cultivated at 17 mM acetate at the entry of stationary phase. Physiological analyses highlight the importance of photosynthesis for the low-acetate and exponential-phase samples. The stationary phase is reached when acetate is depleted, except for the cells grown at 57 mM acetate, which still divide until ammonium exhaustion. Surprisal analysis of the transcriptomics data supports the biological significance of our experiments. This allows the establishment of a model for acetate assimilation, its transcriptional regulation and the identification of candidates for genetic engineering of this metabolic pathway. Altogether, our analyses suggest that growing at high-acetate concentrations could increase biomass productivities in low-light and CO2-limiting air-bubbled medium for biotechnology. View Full-Text
Keywords: Chlamydomonas; growth; acetate concentration; transcriptomics; surprisal analysis Chlamydomonas; growth; acetate concentration; transcriptomics; surprisal analysis
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Bogaert, K.A.; Perez, E.; Rumin, J.; Giltay, A.; Carone, M.; Coosemans, N.; Radoux, M.; Eppe, G.; Levine, R.D.; Remacle, F.; Remacle, C. Metabolic, Physiological, and Transcriptomics Analysis of Batch Cultures of the Green Microalga Chlamydomonas Grown on Different Acetate Concentrations. Cells 2019, 8, 1367.

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