Next Article in Journal
Aquaporins and Their Regulation after Spinal Cord Injury
Next Article in Special Issue
Administration of Tonsil-Derived Mesenchymal Stem Cells Improves Glucose Tolerance in High Fat Diet-Induced Diabetic Mice via Insulin-Like Growth Factor-Binding Protein 5-Mediated Endoplasmic Reticulum Stress Modulation
Previous Article in Journal
Insertion Defects of Mitochondrially Encoded Proteins Burden the Mitochondrial Quality Control System
Open AccessArticle

Subcutaneous Maturation of Neural Stem Cell-Loaded Hydrogels Forms Region-Specific Neuroepithelium

1
Integrated Bioscience Program, The University of Akron, Akron, OH 44325, USA
2
Department of Chemical and Biomolecular Engineering, The University of Akron, Akron, OH 44325, USA
*
Author to whom correspondence should be addressed.
Cells 2018, 7(10), 173; https://doi.org/10.3390/cells7100173
Received: 13 September 2018 / Revised: 9 October 2018 / Accepted: 11 October 2018 / Published: 17 October 2018
(This article belongs to the Special Issue Advances in Stem Cells and Regenerative Medicine)
A combinatorial approach integrating stem cells and capable of exploiting available cues is likely needed to regenerate lost neural tissues and ultimately restore neurologic functions. This study investigates the effects of the subcutaneous maturation of adult-derived neural stem cell (aNSCs) seeded into biomaterial constructs on aNSC differentiation and ultimate regional neuronal identity as a first step toward a future spinal cord injury treatment. To achieve this, we encapsulated rat aNSCs in chitosan-based hydrogels functionalized with immobilized azide-tagged interferon-γ inside a chitosan conduit. Then, we implanted these constructs in the subcutaneous tissues in the backs of rats in the cervical, thoracic, and lumbar regions for 4, 6, and 8 weeks. After harvesting the scaffolds, we analyzed cell differentiation qualitatively using immunohistochemical analysis and quantitatively using RT-qPCR. Results revealed that the hydrogels supported aNSC survival and differentiation up to 4 weeks in the subcutaneous environment as marked by the expression of several neurogenesis markers. Most interesting, the aNSCs expressed region-specific Hox genes corresponding to their region of implantation. This study lays the groundwork for further translational work to recapitulate the potentially undiscovered patterning cues in the subcutaneous tissue and provide support for the conceptual premise that our bioengineering approach can form caudalized region-specific neuroepithelium. View Full-Text
Keywords: chitosan-based scaffold; NSCs; adult-derived neural stem cells; stem cell priming; preconditioning of neural stem cells; region-specific neural tissue; neural identity; instructive biomaterials chitosan-based scaffold; NSCs; adult-derived neural stem cells; stem cell priming; preconditioning of neural stem cells; region-specific neural tissue; neural identity; instructive biomaterials
Show Figures

Figure 1

MDPI and ACS Style

Farrag, M.; Leipzig, N.D. Subcutaneous Maturation of Neural Stem Cell-Loaded Hydrogels Forms Region-Specific Neuroepithelium. Cells 2018, 7, 173.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop