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Complex Commingling: Nucleoporins and the Spindle Assembly Checkpoint

Institute of Molecular Biology and Medicine, Université Libre de Bruxelles, Charleroi 6041, Belgium
Author to whom correspondence should be addressed.
Academic Editor: Alexander E. Kalyuzhny
Cells 2015, 4(4), 706-725;
Received: 19 August 2015 / Revised: 12 October 2015 / Accepted: 28 October 2015 / Published: 3 November 2015
(This article belongs to the Special Issue Nucleocytoplasmic Transport)
PDF [1458 KB, uploaded 4 November 2015]


The segregation of the chromosomes during mitosis is an important process, in which the replicated DNA content is properly allocated into two daughter cells. To ensure their genomic integrity, cells present an essential surveillance mechanism known as the spindle assembly checkpoint (SAC), which monitors the bipolar attachment of the mitotic spindle to chromosomes to prevent errors that would result in chromosome mis-segregation and aneuploidy. Multiple components of the nuclear pore complex (NPC), a gigantic protein complex that forms a channel through the nuclear envelope to allow nucleocytoplasmic exchange of macromolecules, were shown to be critical for faithful cell division and implicated in the regulation of different steps of the mitotic process, including kinetochore and spindle assembly as well as the SAC. In this review, we will describe current knowledge about the interconnection between the NPC and the SAC in an evolutional perspective, which primarily relies on the two mitotic checkpoint regulators, Mad1 and Mad2. We will further discuss the role of NPC constituents, the nucleoporins, in kinetochore and spindle assembly and the formation of the mitotic checkpoint complex during mitosis and interphase. View Full-Text
Keywords: nuclear pore complex; nucleoporins; spindle assembly checkpoint; mitotic checkpoint; mitosis; kinetochore nuclear pore complex; nucleoporins; spindle assembly checkpoint; mitotic checkpoint; mitosis; kinetochore

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).

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Mossaid, I.; Fahrenkrog, B. Complex Commingling: Nucleoporins and the Spindle Assembly Checkpoint. Cells 2015, 4, 706-725.

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