Non-Psychoactive Phytocannabinoids Inhibit Inflammation-Related Changes of Human Coronary Artery Smooth Muscle and Endothelial Cells
Abstract
1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Cellular Viability Assays
2.4. BrdU Proliferation Assay
2.5. Scratch Wound Assay
2.6. Quantitative Reverse Transcriptase Polymerase Chain Reaction (qRT-PCR)
2.7. Total Cellular Protein Isolation
2.8. Nuclear Protein Isolation
2.9. Western Blot Analysis
2.10. Monocyte Adhesion Assay
2.11. Statistics
3. Results
3.1. Nontoxic Concentrations of CBD and THCV Inhibit PDGF-Induced Migration of HCASMC with Additional Inhibition of PDGF-Induced Proliferation by CBD
3.2. CBD and THCV Do Not Impair the Viability of HCAEC at Low Concentrations
3.3. CBD and THCV Elicit Concentration-Dependent Inhibition of IL-1β- and LPS-Induced VCAM-1 but Not ICAM-1 mRNA Levels in HCAEC
3.4. CBD and THCV Cause a Concentration-Dependent Decrease in VCAM-1 but Not ICAM-1 Protein Levels in HCAEC under IL-1β- and LPS-Induced Conditions
3.5. CBD and THCV Reduce VCAM-1 Protein Levels in HCAEC Independently of Cannabinoid Receptors CB1 and CB2, and TRPV1
3.6. p38 MAPK, NF-κB, HDAC, and ROS Mediate IL-1β- and LPS-Induced VCAM-1 Expression in HCAEC, Whereas p42/44 MAPK and JNK Are Not Involved
3.7. CBD and THCV Do Not Cause Inhibition of IL-1β- and LPS-Induced Activation of p38 MAPK
3.8. CBD and THCV Inhibit LPS-, but Not IL-1β-Induced Activation of NF-κB in HCAEC
3.9. CBD Induces Protein Expression of HDAC5 under Stimulated and Unstimulated Conditions in HCAEC
3.10. CBD and THCV Do Not Cause Significant Inhibition of IL-1β- and LPS-Induced Monocyte Adhesion to HCAEC
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Teichmann, E.; Blessing, E.; Hinz, B. Non-Psychoactive Phytocannabinoids Inhibit Inflammation-Related Changes of Human Coronary Artery Smooth Muscle and Endothelial Cells. Cells 2023, 12, 2389. https://doi.org/10.3390/cells12192389
Teichmann E, Blessing E, Hinz B. Non-Psychoactive Phytocannabinoids Inhibit Inflammation-Related Changes of Human Coronary Artery Smooth Muscle and Endothelial Cells. Cells. 2023; 12(19):2389. https://doi.org/10.3390/cells12192389
Chicago/Turabian StyleTeichmann, Elisa, Elane Blessing, and Burkhard Hinz. 2023. "Non-Psychoactive Phytocannabinoids Inhibit Inflammation-Related Changes of Human Coronary Artery Smooth Muscle and Endothelial Cells" Cells 12, no. 19: 2389. https://doi.org/10.3390/cells12192389
APA StyleTeichmann, E., Blessing, E., & Hinz, B. (2023). Non-Psychoactive Phytocannabinoids Inhibit Inflammation-Related Changes of Human Coronary Artery Smooth Muscle and Endothelial Cells. Cells, 12(19), 2389. https://doi.org/10.3390/cells12192389