Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs)
Abstract
1. Introduction
2. Materials and Methods
2.1. Cell Culture
2.2. Peptide Synthesis
2.3. In Vitro Cell Activation
2.4. Mice
2.5. DNBS-Induced Colitis
2.6. Tissue Histology
2.7. Leukocyte Isolation from Colon Lamina Propria
2.8. RNA Isolation and qPCR
2.9. Flow Cytometry
2.10. Immunofluorescence
2.11. Statistical Analysis
3. Results
3.1. Anti-Inflammatory Activity of GILZ Peptides in Human Cell Lines
3.2. Evaluation of NF-κB Nuclear Translocation by Flow Cytometry in Jurkat Cells
3.3. Anti-Inflammatory Activity of PEP-1 in Human Monocytes
3.4. PEP-1 Peptide Administration Ameliorates Clinical Signs in Colitic IL-10KO Mice
3.5. PEP-1 Peptide Treatment Counteracts Leukocytes Infiltration and Prevents NF-κB Nuclear Translocation in Colon Lamina Propria (LP) of IL-10 KO mice
3.6. PEP-1 Protect against the Development of DNBS-Induced Colitis in Mice
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Paglialunga, M.; Flamini, S.; Contini, R.; Febo, M.; Ricci, E.; Ronchetti, S.; Bereshchenko, O.; Migliorati, G.; Riccardi, C.; Bruscoli, S. Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs). Cells 2023, 12, 2294. https://doi.org/10.3390/cells12182294
Paglialunga M, Flamini S, Contini R, Febo M, Ricci E, Ronchetti S, Bereshchenko O, Migliorati G, Riccardi C, Bruscoli S. Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs). Cells. 2023; 12(18):2294. https://doi.org/10.3390/cells12182294
Chicago/Turabian StylePaglialunga, Musetta, Sara Flamini, Raffaele Contini, Marta Febo, Erika Ricci, Simona Ronchetti, Oxana Bereshchenko, Graziella Migliorati, Carlo Riccardi, and Stefano Bruscoli. 2023. "Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs)" Cells 12, no. 18: 2294. https://doi.org/10.3390/cells12182294
APA StylePaglialunga, M., Flamini, S., Contini, R., Febo, M., Ricci, E., Ronchetti, S., Bereshchenko, O., Migliorati, G., Riccardi, C., & Bruscoli, S. (2023). Anti-Inflammatory Effects of Synthetic Peptides Based on Glucocorticoid-Induced Leucine Zipper (GILZ) Protein for the Treatment of Inflammatory Bowel Diseases (IBDs). Cells, 12(18), 2294. https://doi.org/10.3390/cells12182294