Kaempferol and Fisetin-Related Signaling Pathways Induce Apoptosis in Head and Neck Cancer Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Cell Culture
2.3. Fisetin and Kaempferol Treatment
2.4. Cell Proliferation Assay (WST-1 Test)
2.5. Lactate Dehydrogenase Release Test
2.6. Evaluation of Cell Morphology via H&E Cytological Staining
2.7. Evaluation of Apoptosis and Necrosis Using Fluorescence Microscopy
2.8. Flow Cytometry Analysis
2.8.1. FITC Annexin V Apoptosis Detection Kit II
2.8.2. Cell Cycle Analysis—PI/RNase Staining Buffer
2.8.3. Staining Cells with JC-1
2.8.4. PE Active Caspase-3 Apoptosis Kit
2.8.5. PE Anti-Human Bcl-2 Kit
2.9. Cell Migration Test
2.10. Cytochrome c Release Assay
2.11. Statistical Analysis
3. Results
3.1. Kaempferol and Fisetin Inhibit HNCs Cell Proliferation
3.2. Kaempferol and Fisetin Induce Cell Cycle Arrest in SCC-25, SCC-9, and A-253 HNC Cell Lines
3.3. Kaempferol and Fisetin Induce Apoptosis in HNC Cells
3.4. Kaempferol and Fisetin Change the Potential of the Mitochondrial Membrane
3.5. Kaempferol and Fisetin Induce Apoptosis in HNC Cells by Activating Caspase-3
3.6. Kaempferol and Fisetin Inhibit the Anti-Apoptotic Proteins of the Bcl-2 Family
3.7. Fisetin Increases the Release of Cytochrome c in Head and Neck Cancer Cells
3.8. Kaempferol and Fisetin Cause Morphological Changes in HNC Cells
3.9. Kaempferol and Fisetin Induce the Formation of Apoptotic Bodies in HNC Cells
3.10. Kaempferol and Fisetin Inhibit the Migration of HNCs Cells
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kubina, R.; Krzykawski, K.; Dziedzic, A.; Kabała-Dzik, A. Kaempferol and Fisetin-Related Signaling Pathways Induce Apoptosis in Head and Neck Cancer Cells. Cells 2023, 12, 1568. https://doi.org/10.3390/cells12121568
Kubina R, Krzykawski K, Dziedzic A, Kabała-Dzik A. Kaempferol and Fisetin-Related Signaling Pathways Induce Apoptosis in Head and Neck Cancer Cells. Cells. 2023; 12(12):1568. https://doi.org/10.3390/cells12121568
Chicago/Turabian StyleKubina, Robert, Kamil Krzykawski, Arkadiusz Dziedzic, and Agata Kabała-Dzik. 2023. "Kaempferol and Fisetin-Related Signaling Pathways Induce Apoptosis in Head and Neck Cancer Cells" Cells 12, no. 12: 1568. https://doi.org/10.3390/cells12121568