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Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex

Neuroscience Institute, National Research Council (CNR-IN), 35131 Padua, Italy
Department of Biomedical Science, University of Padua, 35131 Padua, Italy
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Dmitri Rusakov
Cells 2022, 11(9), 1400;
Received: 1 April 2022 / Revised: 14 April 2022 / Accepted: 18 April 2022 / Published: 20 April 2022
At glutamatergic synapses, astrocytes respond to the neurotransmitter glutamate with intracellular Ca2+ elevations and the release of gliotransmitters that modulate synaptic transmission. While the functional interactions between neurons and astrocytes have been intensively studied at glutamatergic synapses, the role of astrocytes at GABAergic synapses has been less investigated. In the present study, we combine optogenetics with 2-photon Ca2+ imaging experiments and patch-clamp recording techniques to investigate the signaling between Somatostatin (SST)-releasing GABAergic interneurons and astrocytes in brain slice preparations from the visual cortex (VCx). We found that an intense stimulation of SST interneurons evokes Ca2+ elevations in astrocytes that fundamentally depend on GABAB receptor (GABABR) activation, and that this astrocyte response is modulated by the neuropeptide somatostatin. After episodes of SST interneuron hyperactivity, we also observed a long-lasting reduction of the inhibitory postsynaptic current (IPSC) amplitude onto pyramidal neurons (PNs). This reduction of inhibitory tone (i.e., disinhibition) is counterbalanced by the activation of astrocytes that upregulate SST interneuron-evoked IPSC amplitude by releasing ATP that, after conversion to adenosine, activates A1Rs. Our results describe a hitherto unidentified modulatory mechanism of inhibitory transmission to VCx layer II/III PNs that involves the functional recruitment of astrocytes by SST interneuron signaling. View Full-Text
Keywords: astrocyte; gliotransmission; somatostatin; calcium; disinhibition astrocyte; gliotransmission; somatostatin; calcium; disinhibition
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MDPI and ACS Style

Henriques, V.J.; Chiavegato, A.; Carmignoto, G.; Gómez-Gonzalo, M. Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex. Cells 2022, 11, 1400.

AMA Style

Henriques VJ, Chiavegato A, Carmignoto G, Gómez-Gonzalo M. Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex. Cells. 2022; 11(9):1400.

Chicago/Turabian Style

Henriques, Vanessa Jorge, Angela Chiavegato, Giorgio Carmignoto, and Marta Gómez-Gonzalo. 2022. "Astrocytes Modulate Somatostatin Interneuron Signaling in the Visual Cortex" Cells 11, no. 9: 1400.

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