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Article

Single Cell Effects of Photobiomodulation on Mitochondrial Membrane Potential and Reactive Oxygen Species Production in Human Adipose Mesenchymal Stem Cells

by
Li-Chern Pan
1,†,
Nguyen-Le-Thanh Hang
1,†,
Mamadi M.S Colley
1,
Jungshan Chang
2,
Yu-Cheng Hsiao
1,
Long-Sheng Lu
3,4,5,6,7,8,
Bing-Sian Li
1,
Cheng-Jen Chang
1,9,10,* and
Tzu-Sen Yang
1,4,11,12,*
1
Graduate Institute of Biomedical Optomechatronics, Taipei Medical University, Taipei 110, Taiwan
2
Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
3
Graduate Institute of Biomedical Materials and Tissue Engineering, Taipei Medical University, Taipei 110, Taiwan
4
International Ph.D. Program in Biomedical Engineering, Taipei Medical University, Taipei 110, Taiwan
5
Department of Radiation Oncology, Taipei Medical University Hospital, Taipei Medical University, Taipei 110, Taiwan
6
Department of Medical Research, Taipei Medical University Hospital, Taipei 110, Taiwan
7
TMU Research Center of Cancer Translational Medicine, Taipei Medical University, Taipei 110, Taiwan
8
Center for Cell Therapy, Taipei Medical University Hospital, Taipei Medical University, Taipei 110, Taiwan
9
Department of Plastic Surgery, Taipei Medical University Hospital, Taipei 110, Taiwan
10
Department of Surgery, College of Medicine, Taipei Medical University, Taipei 110, Taiwan
11
School of Dental Technology, Taipei Medical University, Taipei 110, Taiwan
12
Research Center of Biomedical Device, Taipei Medical University, Taipei 110, Taiwan
 
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*
Authors to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Cells 2022, 11(6), 972; https://doi.org/10.3390/cells11060972
Submission received: 10 January 2022 / Revised: 8 March 2022 / Accepted: 9 March 2022 / Published: 11 March 2022
(This article belongs to the Special Issue Single Cell Analysis 2.0)
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Abstract

Photobiomodulation (PBM) has recently emerged in cellular therapy as a potent alternative in promoting cell proliferation, migration, and differentiation during tissue regeneration. Herein, a single-cell near-infrared (NIR) laser irradiation system (830 nm) and the image-based approaches were proposed for the investigation of the modulatory effects in mitochondrial membrane potential (ΔΨm), reactive oxygen species (ROS), and vesicle transport in single living human adipose mesenchymal stem cells (hADSCs). The irradiated-hADSCs were then stained with 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA) and Rhodamine 123 (Rh123) to represent the ΔΨm and ROS production, respectively, with irradiation in the range of 2.5–10 (J/cm2), where time series of bright-field images were obtained to determine the vesicle transport phenomena. Present results showed that a fluence of 5 J/cm2 of PBM significantly enhanced the ΔΨm, ROS, and vesicle transport phenomena compared to the control group (0 J/cm2) after 30 min PBM treatment. These findings demonstrate the efficacy and use of PBM in regulating ΔΨm, ROS, and vesicle transport, which have potential in cell proliferation, migration, and differentiation in cell-based therapy.
Keywords: photobiomodulation; human adipose-derived mesenchymal stem cell; mitochondrial membrane potential; reactive oxygen species; vesicle transport photobiomodulation; human adipose-derived mesenchymal stem cell; mitochondrial membrane potential; reactive oxygen species; vesicle transport
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MDPI and ACS Style

Pan, L.-C.; Hang, N.-L.-T.; Colley, M.M.S.; Chang, J.; Hsiao, Y.-C.; Lu, L.-S.; Li, B.-S.; Chang, C.-J.; Yang, T.-S. Single Cell Effects of Photobiomodulation on Mitochondrial Membrane Potential and Reactive Oxygen Species Production in Human Adipose Mesenchymal Stem Cells. Cells 2022, 11, 972. https://doi.org/10.3390/cells11060972

AMA Style

Pan L-C, Hang N-L-T, Colley MMS, Chang J, Hsiao Y-C, Lu L-S, Li B-S, Chang C-J, Yang T-S. Single Cell Effects of Photobiomodulation on Mitochondrial Membrane Potential and Reactive Oxygen Species Production in Human Adipose Mesenchymal Stem Cells. Cells. 2022; 11(6):972. https://doi.org/10.3390/cells11060972

Chicago/Turabian Style

Pan, Li-Chern, Nguyen-Le-Thanh Hang, Mamadi M.S Colley, Jungshan Chang, Yu-Cheng Hsiao, Long-Sheng Lu, Bing-Sian Li, Cheng-Jen Chang, and Tzu-Sen Yang. 2022. "Single Cell Effects of Photobiomodulation on Mitochondrial Membrane Potential and Reactive Oxygen Species Production in Human Adipose Mesenchymal Stem Cells" Cells 11, no. 6: 972. https://doi.org/10.3390/cells11060972

APA Style

Pan, L.-C., Hang, N.-L.-T., Colley, M. M. S., Chang, J., Hsiao, Y.-C., Lu, L.-S., Li, B.-S., Chang, C.-J., & Yang, T.-S. (2022). Single Cell Effects of Photobiomodulation on Mitochondrial Membrane Potential and Reactive Oxygen Species Production in Human Adipose Mesenchymal Stem Cells. Cells, 11(6), 972. https://doi.org/10.3390/cells11060972

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