Intestinal Epithelial AMPK Deficiency Causes Delayed Colonic Epithelial Repair in DSS-Induced Colitis
Abstract
:1. Introduction
2. Materials and Methods
2.1. Reagents and Antibodies
2.2. Mice
2.3. DSS-Induced Acute Colitis
2.4. Histopathological Analysis
2.5. In Vivo Intestinal Permeability Assay
2.6. Quantification of Lipocalin-2
2.7. Cell Culture and Measure of TEER
2.8. Cell Adhesion Assay and xCELLigence Real-Time Cell Analysis
2.9. Scratch Wound Assay
2.10. Western Blotting
2.11. Quantitative Real-Time PCR Analysis
2.12. Statistical Analysis
3. Results
3.1. IEC-Specific AMPK α1/α2 Deficiency Influenced Intestinal Inflammation upon DSS-Induced Epithelial Injury
3.2. Loss of IEC AMPK α1/α2 Caused Impaired Recovery from DSS-Induced Epithelial Injury
3.3. AMPK Signaling Supports Epithelial Proliferation Following DSS-Mediated Injury
3.4. Absence of AMPK Is Not Detrimental for Cell Spreading Response in Caco2 Cells but Necessary for Mature Barrier Establishment
3.5. Impaired Restitution of Mucus-Producing Goblet Cells in the Colon of IEC AMPK KO Mice Following DSS-Induced Colitis
3.6. Metformin Ameliorated DSS-Induced Colitis in an IEC AMPK-Independent Manner
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Olivier, S.; Diounou, H.; Pochard, C.; Frechin, L.; Durieu, E.; Foretz, M.; Neunlist, M.; Rolli-Derkinderen, M.; Viollet, B. Intestinal Epithelial AMPK Deficiency Causes Delayed Colonic Epithelial Repair in DSS-Induced Colitis. Cells 2022, 11, 590. https://doi.org/10.3390/cells11040590
Olivier S, Diounou H, Pochard C, Frechin L, Durieu E, Foretz M, Neunlist M, Rolli-Derkinderen M, Viollet B. Intestinal Epithelial AMPK Deficiency Causes Delayed Colonic Epithelial Repair in DSS-Induced Colitis. Cells. 2022; 11(4):590. https://doi.org/10.3390/cells11040590
Chicago/Turabian StyleOlivier, Séverine, Hanna Diounou, Camille Pochard, Lisa Frechin, Emilie Durieu, Marc Foretz, Michel Neunlist, Malvyne Rolli-Derkinderen, and Benoit Viollet. 2022. "Intestinal Epithelial AMPK Deficiency Causes Delayed Colonic Epithelial Repair in DSS-Induced Colitis" Cells 11, no. 4: 590. https://doi.org/10.3390/cells11040590