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Volume 11
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10.3390/cells11203207
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Article

Hematopoietic Cells Influence Vascular Development in the Retina

by
Bright Asare-Bediako
1,2,
Yvonne Adu-Agyeiwaah
1,2,
Antonio Abad
3,4,5,
Sergio Li Calzi
2,
Jason L. Floyd
2,
Ram Prasad
2,
Mariana DuPont
1,2,
Richmond Asare-Bediako
6,
Xose R. Bustelo
3 and
Maria B. Grant
2,*
1
Vision Science Graduate Program, School of Optometry, University of Alabama at Birmingham, Birmingham, AL 35233, USA
2
Department of Ophthalmology and Visual Sciences, School of Medicine, University of Alabama, Birmingham, AL 35294, USA
3
Centro de Investigación del Cáncer de Salamanca, CSIC and University of Salamanca, 37007 Salamanca, Spain
4
Instituto de Biología Molecular y Celular del Cáncer, CSIC and University of Salamanca, 37007 Salamanca, Spain
5
Centro de Investigación Biomédica en Red de Cáncer (CIBER), CSIC and University of Salamanca, 37007 Salamanca, Spain
6
Thomas H. Gosnell School of Life Sciences, Rochester Institute of Technology, Rochester, NY 14623, USA
*
Author to whom correspondence should be addressed.
Cells 2022, 11(20), 3207; https://doi.org/10.3390/cells11203207
Submission received: 6 September 2022 / Revised: 4 October 2022 / Accepted: 9 October 2022 / Published: 13 October 2022
(This article belongs to the Special Issue Molecular Mechanisms of Angiogenesis and Inflammation in Retinal Diseases)
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Abstract

Hematopoietic cells play a crucial role in the adult retina in health and disease. Monocytes, macrophages, microglia and myeloid angiogenic cells (MACs) have all been implicated in retinal pathology. However, the role that hematopoietic cells play in retinal development is understudied. The temporal changes in recruitment of hematopoietic cells into the developing retina and the phenotype of the recruited cells are not well understood. In this study, we used the hematopoietic cell-specific protein Vav1 to track and investigate hematopoietic cells in the developing retina. By flow cytometry and immunohistochemistry, we show that hematopoietic cells are present in the retina as early as P0, and include microglia, monocytes and MACs. Even before the formation of retinal blood vessels, hematopoietic cells localize to the inner retina where they eventually form networks that intimately associate with the developing vasculature. Loss of Vav1 lead to a reduction in the density of medium-sized vessels and an increased inflammatory response in retinal astrocytes. When pups were subjected to oxygen-induced retinopathy, hematopoietic cells maintained a close association with the vasculature and occasionally formed ‘frameworks’ for the generation of new vessels. Our study provides further evidence for the underappreciated role of hematopoietic cells in retinal vasculogenesis and the formation of a healthy retina.
Keywords: hematopoietic cells; developing retina; vasculogenesis; microglia; Vav1 knockout hematopoietic cells; developing retina; vasculogenesis; microglia; Vav1 knockout

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MDPI and ACS Style

Asare-Bediako, B.; Adu-Agyeiwaah, Y.; Abad, A.; Li Calzi, S.; Floyd, J.L.; Prasad, R.; DuPont, M.; Asare-Bediako, R.; Bustelo, X.R.; Grant, M.B. Hematopoietic Cells Influence Vascular Development in the Retina. Cells 2022, 11, 3207. https://doi.org/10.3390/cells11203207

AMA Style

Asare-Bediako B, Adu-Agyeiwaah Y, Abad A, Li Calzi S, Floyd JL, Prasad R, DuPont M, Asare-Bediako R, Bustelo XR, Grant MB. Hematopoietic Cells Influence Vascular Development in the Retina. Cells. 2022; 11(20):3207. https://doi.org/10.3390/cells11203207

Chicago/Turabian Style

Asare-Bediako, Bright, Yvonne Adu-Agyeiwaah, Antonio Abad, Sergio Li Calzi, Jason L. Floyd, Ram Prasad, Mariana DuPont, Richmond Asare-Bediako, Xose R. Bustelo, and Maria B. Grant. 2022. "Hematopoietic Cells Influence Vascular Development in the Retina" Cells 11, no. 20: 3207. https://doi.org/10.3390/cells11203207

APA Style

Asare-Bediako, B., Adu-Agyeiwaah, Y., Abad, A., Li Calzi, S., Floyd, J. L., Prasad, R., DuPont, M., Asare-Bediako, R., Bustelo, X. R., & Grant, M. B. (2022). Hematopoietic Cells Influence Vascular Development in the Retina. Cells, 11(20), 3207. https://doi.org/10.3390/cells11203207

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