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Untargeted Multimodal Metabolomics Investigation of the Haemonchus contortus Exsheathment Secretome

1
AgResearch Ltd., Grasslands Research Centre, Palmerston North 4442, New Zealand
2
Laboratory of Functional Helminthology, Institute of Parasitology, Biology Centre of Czech Academy of Science, 37005 Ceske Budejovice, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editors: Sara Rinalducci, María Peña-Chilet and Camilla Hill
Cells 2022, 11(16), 2525; https://doi.org/10.3390/cells11162525
Received: 19 July 2022 / Revised: 11 August 2022 / Accepted: 13 August 2022 / Published: 15 August 2022
(This article belongs to the Special Issue Metabolomics as a Tool for Functional Genomics)
In nematodes that invade the gastro-intestinal tract of the ruminant, the process of larval exsheathment marks the transition from the free-living to the parasitic stages of these parasites. To investigate the secretome associated with larval exsheathment, a closed in vitro system that effectively reproduces the two basic components of an anaerobic rumen environment (CO2 and 39 °C) was developed to trigger exsheathment in one of the most pathogenic and model gastrointestinal parasitic nematodes, Haemonchus contortus (barber‘s pole worm). This study reports the use of multimodal untargeted metabolomics and lipidomics methodologies to identify the metabolic signatures and compounds secreted during in vitro larval exsheathment in the H. contortus infective third-stage larva (iL3). A combination of statistical and chemoinformatic analyses using three analytical platforms revealed a panel of metabolites detected post exsheathment and associated with amino acids, purines, as well as select organic compounds. The major lipid classes identified by the non-targeted lipidomics method applied were lysophosphatidylglycerols, diglycerides, fatty acyls, glycerophospholipids, and a triglyceride. The identified metabolites may serve as metabolic signatures to improve tractability of parasitic nematodes for characterizing small molecule host–parasite interactions related to pathogenesis, vaccine and drug design, as well as the discovery of metabolic biomarkers. View Full-Text
Keywords: Haemonchus contortus; metabolomics; lipidomics; helminth; parasite; exsheathment Haemonchus contortus; metabolomics; lipidomics; helminth; parasite; exsheathment
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MDPI and ACS Style

Palevich, N.; Maclean, P.H.; Candy, P.M.; Taylor, W.; Mladineo, I.; Cao, M. Untargeted Multimodal Metabolomics Investigation of the Haemonchus contortus Exsheathment Secretome. Cells 2022, 11, 2525. https://doi.org/10.3390/cells11162525

AMA Style

Palevich N, Maclean PH, Candy PM, Taylor W, Mladineo I, Cao M. Untargeted Multimodal Metabolomics Investigation of the Haemonchus contortus Exsheathment Secretome. Cells. 2022; 11(16):2525. https://doi.org/10.3390/cells11162525

Chicago/Turabian Style

Palevich, Nikola, Paul H. Maclean, Paul M. Candy, Wendy Taylor, Ivona Mladineo, and Mingshu Cao. 2022. "Untargeted Multimodal Metabolomics Investigation of the Haemonchus contortus Exsheathment Secretome" Cells 11, no. 16: 2525. https://doi.org/10.3390/cells11162525

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