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Article

Differential Expression of miRNAs and Their Predicted Target Pathways in Cochlear Nucleus Following Chronic Noise Exposure in Rats

Department of Otorhinolaryngology-Head & Neck Surgery, College of Medicine, CHA University, Seongnam 13496, Korea
*
Author to whom correspondence should be addressed.
Academic Editor: Daniele Nosi
Cells 2022, 11(15), 2266; https://doi.org/10.3390/cells11152266
Received: 26 May 2022 / Revised: 20 July 2022 / Accepted: 20 July 2022 / Published: 22 July 2022
(This article belongs to the Special Issue Frontiers in Neuroinflammation)
Several recent preclinical studies have reported that dynamic changes in miRNA expression contribute to hearing function. This study aims to investigate miRNA expression changes in the cochlear nuclei (CN) of rats following chronic noise exposure. Eight-week-old rats (n = 14) were exposed to noise for 4 weeks. The control rats (n = 14) were raised under identical conditions without noise. Two months after noise exposure, the auditory brainstem response (ABR) was examined, and the cochlea and CN were harvested. In the CN, the expression levels of arc, neurocan, and brevican were measured (n = 6 per group). Furthermore, the expression levels of miRNAs and their predicted target genes were measured in the CN (n = 8 per group). ABR thresholds were elevated after 4 weeks of noise exposure, which were maintained for 3 months. In CN, the protein expression of arc and brevican was higher in the noise-exposed group than in the control group (0.95 [standard deviation (SD) = 0.53] vs. 3.19 [SD = 1.00], p < 0.001 for arc and 1.02 [SD = 0.10] vs. 1.66 [SD = 0.24], p < 0.001 for brevican). The noise-exposed rats exhibited lower expression levels of miR-758-5p, miR-15b-5p, miR-212-3p, miR-199a-5p, and miR-134-3p than the control rats (all p < 0.001). The AMPK signaling pathway was predicted to be regulated by these miRNAs. The predicted target genes AKT3, SIRT1, and PRKAA1 were highly expressed in noise-exposed rats. In CN of noise-exposed rats, the miRNAs of miR-758-5p, miR-15b-5p, miR-212-3p, miR-199a-5p, and miR-134-3p were reduced and related to AMPK signaling including AKT3 and SIRT1 expression. These modulation of signaling pathways could mediate the increased expression of brevican in the CN of noise-exposed rats. View Full-Text
Keywords: microRNAs; noise; hearing loss; cochlear nucleus; AKT3; SIRT1 microRNAs; noise; hearing loss; cochlear nucleus; AKT3; SIRT1
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MDPI and ACS Style

Lee, C.H.; Jeon, J.; Lee, S.M.; Kim, S.Y. Differential Expression of miRNAs and Their Predicted Target Pathways in Cochlear Nucleus Following Chronic Noise Exposure in Rats. Cells 2022, 11, 2266. https://doi.org/10.3390/cells11152266

AMA Style

Lee CH, Jeon J, Lee SM, Kim SY. Differential Expression of miRNAs and Their Predicted Target Pathways in Cochlear Nucleus Following Chronic Noise Exposure in Rats. Cells. 2022; 11(15):2266. https://doi.org/10.3390/cells11152266

Chicago/Turabian Style

Lee, Chang Ho, Jiwon Jeon, So Min Lee, and So Young Kim. 2022. "Differential Expression of miRNAs and Their Predicted Target Pathways in Cochlear Nucleus Following Chronic Noise Exposure in Rats" Cells 11, no. 15: 2266. https://doi.org/10.3390/cells11152266

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