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Article

Isolation and Characterization of Tissue Resident CD29-Positive Progenitor Cells in Livestock to Generate a Three-Dimensional Meat Bud

1
Intractable Disease Research Center, Juntendo University School of Medicine, Tokyo 113-8421, Japan
2
Department of Biochemistry and Biophysics, Graduate School of Medical and Dental Sciences, Tokyo Medical and Dental University (TMDU), Tokyo 1130-8510, Japan
3
Institute of Research, Tokyo Medical and Dental University (TMDU), Tokyo 113-8510, Japan
4
Department of Research and Development for Organoids, Juntendo University School of Medicine, Tokyo 113-8421, Japan
5
Division of Gastroenterology, Hepatology and Nutrition, Division of Developmental Biology, Center for Stem Cell and Organoid Medicine (CuSTOM), Cincinnati Children Hospital Medical Center, Cincinnati, OH 45229-3039, USA
*
Author to whom correspondence should be addressed.
Yuna Naraoka and Yo Mabuchi contributed equally to this work.
Academic Editor: Kunimasa Ohta
Cells 2021, 10(9), 2499; https://doi.org/10.3390/cells10092499
Received: 2 August 2021 / Revised: 15 September 2021 / Accepted: 16 September 2021 / Published: 21 September 2021
(This article belongs to the Special Issue Signaling Pathways in Cell Generation and Reprogramming)
The current process of meat production using livestock has significant effects on the global environment, including high emissions of greenhouse gases. In recent years, cultured meat has attracted attention as a way to acquire animal proteins. However, the lack of markers that isolate proliferating cells from bovine tissues and the complex structure of the meat make it difficult to culture meat in a dish. In this study, we screened 246 cell-surface antibodies by fluorescence-activated cell sorting for their capacity to form colonies and their suitability to construct spheroid “meat buds”. CD29+ cells (Ha2/5 clone) have a high potency to form colonies and efficiently proliferate on fibronectin-coated dishes. Furthermore, the meat buds created from CD29+ cells could differentiate into muscle and adipose cells in a three-dimensional structure. The meat buds embedded in the collagen gel proliferated in the matrix and formed large aggregates. Approximately 10 trillion cells can theoretically be obtained from 100 g of bovine tissue by culturing and amplifying them using these methods. The CD29+ cell characteristics of bovine tissue provide insights into the production of meat alternatives in vitro. View Full-Text
Keywords: CD29; Ha2/5; mesenchymal stem/stromal cells; adipogenic differentiation; culture meat; flow cytometry CD29; Ha2/5; mesenchymal stem/stromal cells; adipogenic differentiation; culture meat; flow cytometry
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MDPI and ACS Style

Naraoka, Y.; Mabuchi, Y.; Yoneyama, Y.; Suto, E.G.; Hisamatsu, D.; Ikeda, M.; Ito, R.; Nakamura, T.; Takebe, T.; Akazawa, C. Isolation and Characterization of Tissue Resident CD29-Positive Progenitor Cells in Livestock to Generate a Three-Dimensional Meat Bud. Cells 2021, 10, 2499. https://doi.org/10.3390/cells10092499

AMA Style

Naraoka Y, Mabuchi Y, Yoneyama Y, Suto EG, Hisamatsu D, Ikeda M, Ito R, Nakamura T, Takebe T, Akazawa C. Isolation and Characterization of Tissue Resident CD29-Positive Progenitor Cells in Livestock to Generate a Three-Dimensional Meat Bud. Cells. 2021; 10(9):2499. https://doi.org/10.3390/cells10092499

Chicago/Turabian Style

Naraoka, Yuna, Yo Mabuchi, Yosuke Yoneyama, Eriko G. Suto, Daisuke Hisamatsu, Mami Ikeda, Risa Ito, Tetsuya Nakamura, Takanori Takebe, and Chihiro Akazawa. 2021. "Isolation and Characterization of Tissue Resident CD29-Positive Progenitor Cells in Livestock to Generate a Three-Dimensional Meat Bud" Cells 10, no. 9: 2499. https://doi.org/10.3390/cells10092499

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