Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo
1
Department of Health Science, School of Biomedical Sciences, Universidad Europea de Madrid, 28670 Villaviciosa de Odón, Spain
2
Department of Medicine, School of Biomedical Sciences, Universidad Europea de Madrid, 28670 Villaviciosa de Odón, Spain
3
Department of Dentistry, School of Biomedical Sciences, Universidad Europea de Madrid, 28670 Villaviciosa de Odón, Spain
4
Vascular Pathophysiology Department, Centro Nacional de Investigaciones Cardiovasculares (CNIC-CSIC), 28029 Madrid, Spain
5
Molecular Biomedicine Department, Centro de Investigaciones Biológicas Margarita Salas (CIB-CSIC), 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editors: Stephen Yarwood and Charles J. Malemud
Cells 2021, 10(9), 2448; https://doi.org/10.3390/cells10092448
Received: 28 July 2021
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Revised: 3 September 2021
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Accepted: 15 September 2021
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Published: 17 September 2021
(This article belongs to the Section Cell Signaling)
MT1-MMP/MMP14 belongs to a subgroup of the matrix metalloproteinases family that presents a transmembrane domain, with a cytosolic tail and the catalytic site exposed to the extracellular space. Deficient mice for this enzyme result in early postnatal death and display severe defects in skeletal, muscle and lung development. By using a transgenic line expressing the LacZ reporter under the control of the endogenous Mt1-mmp promoter, we reported a dynamic spatiotemporal expression pattern for Mt1-mmp from early embryonic to perinatal stages during cardiovascular development and brain formation. Thus, Mt1-mmp shows expression in the endocardium of the heart and the truncus arteriosus by E8.5, and is also strongly detected during vascular system development as well as in endothelial cells. In the brain, LacZ reporter expression was detected in the olfactory bulb, the rostral cerebral cortex and the caudal mesencephalic tectum. LacZ-positive cells were observed in neural progenitors of the spinal cord, neural crest cells and the intersomitic region. In the limb, Mt1-mmp expression was restricted to blood vessels, cartilage primordium and muscles. Detection of the enzyme was confirmed by Western blot and immunohistochemical analysis. We suggest novel functions for this metalloproteinase in angiogenesis, endocardial formation and vascularization during organogenesis. Moreover, Mt1-mmp expression revealed that the enzyme may contribute to heart, muscle and brain throughout development.
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Keywords:
Mt1-mmp; MMP14; metalloproteinases; embryo development; angiogenesis; expression pattern; cardiovascular development; brain; limb; dorsal aorta
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MDPI and ACS Style
Muñoz-Sáez, E.; Moracho, N.; Learte, A.I.R.; Arroyo, A.G.; Sánchez-Camacho, C. Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo. Cells 2021, 10, 2448. https://doi.org/10.3390/cells10092448
AMA Style
Muñoz-Sáez E, Moracho N, Learte AIR, Arroyo AG, Sánchez-Camacho C. Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo. Cells. 2021; 10(9):2448. https://doi.org/10.3390/cells10092448
Chicago/Turabian StyleMuñoz-Sáez, Emma, Natalia Moracho, Ana I. R. Learte, Alicia G. Arroyo, and Cristina Sánchez-Camacho. 2021. "Dynamic Expression of Membrane Type 1-Matrix Metalloproteinase (Mt1-mmp/Mmp14) in the Mouse Embryo" Cells 10, no. 9: 2448. https://doi.org/10.3390/cells10092448
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