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Article

Transcriptomic Response Dynamics of Human Primary and Immortalized Adrenocortical Cells to Steroidogenic Stimuli

1
RNA Bioscience Initiative, University of Colorado School of Medicine, Aurora, CO 80045, USA
2
Department of Biochemistry and Molecular Genetics, University of Colorado School of Medicine, Aurora, CO 80045, USA
3
Department of Molecular and Integrative Physiology, University of Michigan School of Medicine, Ann Arbor, MI 48109, USA
*
Author to whom correspondence should be addressed.
Authors made equal contributions.
Academic Editor: Marcin Rucinski
Cells 2021, 10(9), 2376; https://doi.org/10.3390/cells10092376
Received: 20 July 2021 / Revised: 27 August 2021 / Accepted: 6 September 2021 / Published: 9 September 2021
Adrenal steroid hormone production is a dynamic process stimulated by adrenocorticotropic hormone (ACTH) and angiotensin II (AngII). These ligands initialize a rapid and robust gene expression response required for steroidogenesis. Here, we compare the predominant human immortalized cell line model, H295R cell, with primary cultures of adult adrenocortical cells derived from human kidney donors. We performed temporally resolved RNA-seq on primary cells stimulated with either ACTH or AngII at multiple time points. The magnitude of the expression dynamics elicited by ACTH was greater than AngII in primary cells. This is likely due to the larger population of adrenocortical cells that are responsive to ACTH. The dynamics of stimulus-induced expression in H295R cells are mostly recapitulated in primary cells. However, there are some expression responses in primary cells absent in H295R cells. These data are a resource for the endocrine community and will help researchers determine whether H295R is an appropriate model for the specific aspect of steroidogenesis that they are studying. View Full-Text
Keywords: adrenal; steroid hormones; stimulus response; ACTH; angiotensin II adrenal; steroid hormones; stimulus response; ACTH; angiotensin II
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MDPI and ACS Style

Wellman, K.; Fu, R.; Baldwin, A.; Rege, J.; Murphy, E.; Rainey, W.E.; Mukherjee, N. Transcriptomic Response Dynamics of Human Primary and Immortalized Adrenocortical Cells to Steroidogenic Stimuli. Cells 2021, 10, 2376. https://doi.org/10.3390/cells10092376

AMA Style

Wellman K, Fu R, Baldwin A, Rege J, Murphy E, Rainey WE, Mukherjee N. Transcriptomic Response Dynamics of Human Primary and Immortalized Adrenocortical Cells to Steroidogenic Stimuli. Cells. 2021; 10(9):2376. https://doi.org/10.3390/cells10092376

Chicago/Turabian Style

Wellman, Kimberly, Rui Fu, Amber Baldwin, Juilee Rege, Elisabeth Murphy, William E. Rainey, and Neelanjan Mukherjee. 2021. "Transcriptomic Response Dynamics of Human Primary and Immortalized Adrenocortical Cells to Steroidogenic Stimuli" Cells 10, no. 9: 2376. https://doi.org/10.3390/cells10092376

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