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Article

Transcriptome and Methylome Analysis Reveal Complex Cross-Talks between Thyroid Hormone and Glucocorticoid Signaling at Xenopus Metamorphosis

1
UMR7221 Molecular Physiology and Adaption, CNRS, Museum National d’Histoire Naturelle, 57 Rue Cuvier, CEDEX 05, 75231 Paris, France
2
Genomics Core Facility, Département de Biologie, Institut de Biologie de l’ENS (IBENS), École Normale Supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France
*
Author to whom correspondence should be addressed.
Co-first authors, alphabetic order.
Academic Editor: Michelina Plateroti
Cells 2021, 10(9), 2375; https://doi.org/10.3390/cells10092375
Received: 15 July 2021 / Revised: 31 August 2021 / Accepted: 3 September 2021 / Published: 9 September 2021
Background: Most work in endocrinology focus on the action of a single hormone, and very little on the cross-talks between two hormones. Here we characterize the nature of interactions between thyroid hormone and glucocorticoid signaling during Xenopus tropicalis metamorphosis. Methods: We used functional genomics to derive genome wide profiles of methylated DNA and measured changes of gene expression after hormonal treatments of a highly responsive tissue, tailfin. Clustering classified the data into four types of biological responses, and biological networks were modeled by system biology. Results: We found that gene expression is mostly regulated by either T3 or CORT, or their additive effect when they both regulate the same genes. A small but non-negligible fraction of genes (12%) displayed non-trivial regulations indicative of complex interactions between the signaling pathways. Strikingly, DNA methylation changes display the opposite and are dominated by cross-talks. Conclusion: Cross-talks between thyroid hormones and glucocorticoids are more complex than initially envisioned and are not limited to the simple addition of their individual effects, a statement that can be summarized with the pseudo-equation: TH GC > TH + GC. DNA methylation changes are highly dynamic and buffered from genome expression. View Full-Text
Keywords: Xenopus metamorphosis; thyroid hormone; glucocorticoids; cross-talks; functional genomics; DNA methylation Xenopus metamorphosis; thyroid hormone; glucocorticoids; cross-talks; functional genomics; DNA methylation
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MDPI and ACS Style

Buisine, N.; Grimaldi, A.; Jonchere, V.; Rigolet, M.; Blugeon, C.; Hamroune, J.; Sachs, L.M. Transcriptome and Methylome Analysis Reveal Complex Cross-Talks between Thyroid Hormone and Glucocorticoid Signaling at Xenopus Metamorphosis. Cells 2021, 10, 2375. https://doi.org/10.3390/cells10092375

AMA Style

Buisine N, Grimaldi A, Jonchere V, Rigolet M, Blugeon C, Hamroune J, Sachs LM. Transcriptome and Methylome Analysis Reveal Complex Cross-Talks between Thyroid Hormone and Glucocorticoid Signaling at Xenopus Metamorphosis. Cells. 2021; 10(9):2375. https://doi.org/10.3390/cells10092375

Chicago/Turabian Style

Buisine, Nicolas, Alexis Grimaldi, Vincent Jonchere, Muriel Rigolet, Corinne Blugeon, Juliette Hamroune, and Laurent M. Sachs. 2021. "Transcriptome and Methylome Analysis Reveal Complex Cross-Talks between Thyroid Hormone and Glucocorticoid Signaling at Xenopus Metamorphosis" Cells 10, no. 9: 2375. https://doi.org/10.3390/cells10092375

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