Lipid Polymorphism of the Subchloroplast—Granum and Stroma Thylakoid Membrane—Particles. I. 31P-NMR Spectroscopy
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation of Granum and Stroma Thylakoid Membranes
2.2. Lipase Treatments
2.3. 31P-NMR Measurements
3. Results
3.1. 31P-NMR Fingerprints of Lipid Phases in Isolated Granum and Stroma Thylakoid Membranes
3.2. Saturation-Transfer Experiments
3.3. Temperature Dependences
3.4. Effect of Wheat Germ Lipase
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Granum | Stroma | |||
---|---|---|---|---|
FWHM | Position (ppm) | FWHM | Position (ppm) | |
I1 | 1.22 ± 0.16 | 2.66 ± 0.07 | 0.71 ± 0.10 | 2.68 ± 0.16 |
I2 | 1.51 ± 0.96 | 3.98 ± 0.40 | 0.89 ± 0.13 | 4.19 ± 0.13 |
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Dlouhý, O.; Javornik, U.; Zsiros, O.; Šket, P.; Karlický, V.; Špunda, V.; Plavec, J.; Garab, G. Lipid Polymorphism of the Subchloroplast—Granum and Stroma Thylakoid Membrane—Particles. I. 31P-NMR Spectroscopy. Cells 2021, 10, 2354. https://doi.org/10.3390/cells10092354
Dlouhý O, Javornik U, Zsiros O, Šket P, Karlický V, Špunda V, Plavec J, Garab G. Lipid Polymorphism of the Subchloroplast—Granum and Stroma Thylakoid Membrane—Particles. I. 31P-NMR Spectroscopy. Cells. 2021; 10(9):2354. https://doi.org/10.3390/cells10092354
Chicago/Turabian StyleDlouhý, Ondřej, Uroš Javornik, Ottó Zsiros, Primož Šket, Václav Karlický, Vladimír Špunda, Janez Plavec, and Győző Garab. 2021. "Lipid Polymorphism of the Subchloroplast—Granum and Stroma Thylakoid Membrane—Particles. I. 31P-NMR Spectroscopy" Cells 10, no. 9: 2354. https://doi.org/10.3390/cells10092354