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Article

Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes

by
Marta Gomez-Perosanz
1,
Tara Fiyouzi
1,
Miguel Fernandez-Arquero
2,
John Sidney
3,
Alessandro Sette
3,
Ellis L. Reinherz
4,
Esther M. Lafuente
1 and
Pedro A. Reche
1,*
1
Department of Immunology, School of Medicine, Complutense University of Madrid, 28040 Madrid, Spain
2
Immunology Service, San Carlos University Hospital, 28040 Madrid, Spain
3
Division of Vaccine Discovery, La Jolla Institute for Immunology, La Jolla, CA 92037, USA
4
Laboratory of Immunobiology, Dana-Farber Cancer Institute, Boston, MA 02215, USA
*
Author to whom correspondence should be addressed.
Cells 2021, 10(9), 2294; https://doi.org/10.3390/cells10092294
Submission received: 30 July 2021 / Revised: 26 August 2021 / Accepted: 31 August 2021 / Published: 2 September 2021
(This article belongs to the Collection Advances in Immune Monitoring)
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Abstract

Human rhinovirus (RV) is the most common cause of upper respiratory infections and exacerbations of asthma. In this work, we selected 14 peptides (6 from RV A and 8 from RV C) encompassing potential CD4 T cell epitopes. Peptides were selected for being highly conserved in RV A and C serotypes and predicted to bind to multiple human leukocyte antigen class II (HLA II) molecules. We found positive T cell recall responses by interferon gamma (IFNγ)-ELISPOT assays to eight peptides, validating seven of them (three from RV A and four from RV C) as CD4 T cell epitopes through intracellular cytokine staining assays. Additionally, we verified their promiscuous binding to multiple HLA II molecules by quantitative binding assays. According to their experimental HLA II binding profile, the combination of all these seven epitopes could be recognized by >95% of the world population. We actually determined IFNγ responses to a pool encompassing these CD4 T cell epitopes by intracellular cytokine staining, finding positive responses in 29 out of 30 donors. The CD4 T cell epitopes identified in this study could be key to monitor RV infections and to develop peptide-based vaccines against most RV A and C serotypes.
Keywords: Human rhinovirus; CD4 T cell; epitope; peptide Human rhinovirus; CD4 T cell; epitope; peptide

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MDPI and ACS Style

Gomez-Perosanz, M.; Fiyouzi, T.; Fernandez-Arquero, M.; Sidney, J.; Sette, A.; Reinherz, E.L.; Lafuente, E.M.; Reche, P.A. Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes. Cells 2021, 10, 2294. https://doi.org/10.3390/cells10092294

AMA Style

Gomez-Perosanz M, Fiyouzi T, Fernandez-Arquero M, Sidney J, Sette A, Reinherz EL, Lafuente EM, Reche PA. Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes. Cells. 2021; 10(9):2294. https://doi.org/10.3390/cells10092294

Chicago/Turabian Style

Gomez-Perosanz, Marta, Tara Fiyouzi, Miguel Fernandez-Arquero, John Sidney, Alessandro Sette, Ellis L. Reinherz, Esther M. Lafuente, and Pedro A. Reche. 2021. "Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes" Cells 10, no. 9: 2294. https://doi.org/10.3390/cells10092294

APA Style

Gomez-Perosanz, M., Fiyouzi, T., Fernandez-Arquero, M., Sidney, J., Sette, A., Reinherz, E. L., Lafuente, E. M., & Reche, P. A. (2021). Characterization of Conserved and Promiscuous Human Rhinovirus CD4 T Cell Epitopes. Cells, 10(9), 2294. https://doi.org/10.3390/cells10092294

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