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Article

PopulusPtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen

1
Umeå Plant Science Centre, Department of Plant Physiology, Umeå University, SE-90187 Umeå, Sweden
2
Organismal and Evolutionary Biology Research Programme, Faculty of Biological and Environmental Sciences, University of Helsinki, FI-00014 Helsinki, Finland
3
Umeå Plant Science Centre, Department of Forest Genetics and Plant Physiology, Swedish University of Agricultural Sciences, SE-90184 Umeå, Sweden
4
Faculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, 1433 Ås, Norway
*
Author to whom correspondence should be addressed.
Current address: VIB Center for Plant Systems Biology, 9052 Ghent, Belgium.
Academic Editors: Elisabeth Jamet and Christophe Dunand
Cells 2021, 10(8), 1971; https://doi.org/10.3390/cells10081971
Received: 7 June 2021 / Revised: 23 July 2021 / Accepted: 27 July 2021 / Published: 3 August 2021
(This article belongs to the Special Issue Research on Plant Cell Wall Biology)
Secondary growth relies on precise and specialized transcriptional networks that determine cell division, differentiation, and maturation of xylem cells. We identified a novel role for the ethylene-induced Populus Ethylene Response Factor PtERF85 (Potri.015G023200) in balancing xylem cell expansion and secondary cell wall (SCW) formation in hybrid aspen (Populus tremula x tremuloides). Expression of PtERF85 is high in phloem and cambium cells and during the expansion of xylem cells, while it is low in maturing xylem tissue. Extending PtERF85 expression into SCW forming zones of woody tissues through ectopic expression reduced wood density and SCW thickness of xylem fibers but increased fiber diameter. Xylem transcriptomes from the transgenic trees revealed transcriptional induction of genes involved in cell expansion, translation, and growth. The expression of genes associated with plant vascular development and the biosynthesis of SCW chemical components such as xylan and lignin, was down-regulated in the transgenic trees. Our results suggest that PtERF85 activates genes related to xylem cell expansion, while preventing transcriptional activation of genes related to SCW formation. The importance of precise spatial expression of PtERF85 during wood development together with the observed phenotypes in response to ectopic PtERF85 expression suggests that PtERF85 contributes to the transition of fiber cells from elongation to secondary cell wall deposition. View Full-Text
Keywords: cell wall thickness; ERF85 (CRF4); lignin; ribosome biogenesis; wood development; xylem expansion cell wall thickness; ERF85 (CRF4); lignin; ribosome biogenesis; wood development; xylem expansion
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MDPI and ACS Style

Seyfferth, C.; Wessels, B.A.; Vahala, J.; Kangasjärvi, J.; Delhomme, N.; Hvidsten, T.R.; Tuominen, H.; Lundberg-Felten, J. PopulusPtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen. Cells 2021, 10, 1971. https://doi.org/10.3390/cells10081971

AMA Style

Seyfferth C, Wessels BA, Vahala J, Kangasjärvi J, Delhomme N, Hvidsten TR, Tuominen H, Lundberg-Felten J. PopulusPtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen. Cells. 2021; 10(8):1971. https://doi.org/10.3390/cells10081971

Chicago/Turabian Style

Seyfferth, Carolin, Bernard A. Wessels, Jorma Vahala, Jaakko Kangasjärvi, Nicolas Delhomme, Torgeir R. Hvidsten, Hannele Tuominen, and Judith Lundberg-Felten. 2021. "PopulusPtERF85 Balances Xylem Cell Expansion and Secondary Cell Wall Formation in Hybrid Aspen" Cells 10, no. 8: 1971. https://doi.org/10.3390/cells10081971

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