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Differential Tropism in Roots and Shoots of Resistant and Susceptible Cassava (Manihot esculenta Crantz) Infected by Cassava Brown Streak Viruses

Plant Virus Department, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, 38124 Braunschweig, Germany
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Author to whom correspondence should be addressed.
Academic Editor: Suleyman Allakhverdiev
Cells 2021, 10(5), 1221; https://doi.org/10.3390/cells10051221
Received: 27 April 2021 / Revised: 12 May 2021 / Accepted: 13 May 2021 / Published: 17 May 2021
(This article belongs to the Section Plant, Algae and Fungi Cell Biology)
Cassava brown streak disease (CBSD) is a destructive disease of cassava in Eastern and Central Africa. Because there was no source of resistance in African varieties to provide complete protection against the viruses causing the disease, we searched in South American germplasm and identified cassava lines that did not become infected with the cassava brown streak viruses. These findings motivated further investigations into the mechanism of virus resistance. We used RNAscope® in situ hybridization to localize cassava brown streak virus in cassava germplasm lines that were highly resistant (DSC 167, immune) or that restricted virus infections to stems and roots only (DSC 260). We show that the resistance in those lines is not a restriction of long-distance movement but due to preventing virus unloading from the phloem into parenchyma cells for replication, thus restricting the virus to the phloem cells only. When DSC 167 and DSC 260 were compared for virus invasion, only a low CBSV signal was found in phloem tissue of DSC 167, indicating that there is no replication in this host, while the presence of intense hybridization signals in the phloem of DSC 260 provided evidence for virus replication in companion cells. In neither of the two lines studied was there evidence of virus replication outside the phloem tissues. Thus, we conclude that in resistant cassava lines, CBSV is confined to the phloem tissues only, in which virus replication can still take place or is arrested. View Full-Text
Keywords: Ipomovirus; virus resistance; phloem unloading; companion cells; tissue localization; cryo-sectioning; RNAscope® ISH Ipomovirus; virus resistance; phloem unloading; companion cells; tissue localization; cryo-sectioning; RNAscope® ISH
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MDPI and ACS Style

Sheat, S.; Margaria, P.; Winter, S. Differential Tropism in Roots and Shoots of Resistant and Susceptible Cassava (Manihot esculenta Crantz) Infected by Cassava Brown Streak Viruses. Cells 2021, 10, 1221. https://doi.org/10.3390/cells10051221

AMA Style

Sheat S, Margaria P, Winter S. Differential Tropism in Roots and Shoots of Resistant and Susceptible Cassava (Manihot esculenta Crantz) Infected by Cassava Brown Streak Viruses. Cells. 2021; 10(5):1221. https://doi.org/10.3390/cells10051221

Chicago/Turabian Style

Sheat, Samar, Paolo Margaria, and Stephan Winter. 2021. "Differential Tropism in Roots and Shoots of Resistant and Susceptible Cassava (Manihot esculenta Crantz) Infected by Cassava Brown Streak Viruses" Cells 10, no. 5: 1221. https://doi.org/10.3390/cells10051221

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