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Article

Metabolomic Analysis Evidences That Uterine Epithelial Cells Enhance Blastocyst Development in a Microfluidic Device

1
School of Electronic and Electrical Engineering, University of Leeds, Leeds LS2 9JT, UK
2
Center for Innovative Technology (CIT), Department of Chemistry, Vanderbilt University, 7300 Stevenson Center Lane, Nashville, TN 37235, USA
3
Reproduction and Early Development Research Group, Discovery and Translational Science Department, Leeds Institute of Cardiovascular and Metabolic Medicine, School of Medicine, University of Leeds, Leeds LS2 9JT, UK
4
Leeds Institute of Medical Research, University of Leeds, Leeds LS2 9JT, UK
*
Author to whom correspondence should be addressed.
Academic Editors: Barbara Barboni, Valentina Russo and Nicola Bernabò
Cells 2021, 10(5), 1194; https://doi.org/10.3390/cells10051194
Received: 14 April 2021 / Revised: 9 May 2021 / Accepted: 11 May 2021 / Published: 13 May 2021
Here we report the use of a microfluidic system to assess the differential metabolomics of murine embryos cultured with endometrial cells-conditioned media (CM). Groups of 10, 1-cell murine B6C3F1 × B6D2F1 embryos were cultured in the microfluidic device. To produce CM, mouse uterine epithelial cells were cultured in potassium simplex optimized medium (KSOM) for 24 h. Media samples were collected from devices after 5 days of culture with KSOM (control) and CM, analyzed by reverse phase liquid chromatography and untargeted positive ion mode mass spectrometry analysis. Blastocyst rates were significantly higher (p < 0.05) in CM (71.8%) compared to control media (54.6%). We observed significant upregulation of 341 compounds and downregulation of 214 compounds in spent media from CM devices when compared to control. Out of these, 353 compounds were identified showing a significant increased abundance of metabolites involved in key metabolic pathways (e.g., arginine, proline and pyrimidine metabolism) in the CM group, suggesting a beneficial effect of CM on embryo development. The metabolomic study carried out in a microfluidic environment confirms our hypothesis on the potential of uterine epithelial cells to enhance blastocyst development. Further investigations are required to highlight specific pathways involved in embryo development and implantation. View Full-Text
Keywords: embryo culture; microfluidics; metabolomics embryo culture; microfluidics; metabolomics
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MDPI and ACS Style

Mancini, V.; Schrimpe-Rutledge, A.C.; Codreanu, S.G.; Sherrod, S.D.; McLean, J.A.; Picton, H.M.; Pensabene, V. Metabolomic Analysis Evidences That Uterine Epithelial Cells Enhance Blastocyst Development in a Microfluidic Device. Cells 2021, 10, 1194. https://doi.org/10.3390/cells10051194

AMA Style

Mancini V, Schrimpe-Rutledge AC, Codreanu SG, Sherrod SD, McLean JA, Picton HM, Pensabene V. Metabolomic Analysis Evidences That Uterine Epithelial Cells Enhance Blastocyst Development in a Microfluidic Device. Cells. 2021; 10(5):1194. https://doi.org/10.3390/cells10051194

Chicago/Turabian Style

Mancini, Vanessa, Alexandra C. Schrimpe-Rutledge, Simona G. Codreanu, Stacy D. Sherrod, John A. McLean, Helen M. Picton, and Virginia Pensabene. 2021. "Metabolomic Analysis Evidences That Uterine Epithelial Cells Enhance Blastocyst Development in a Microfluidic Device" Cells 10, no. 5: 1194. https://doi.org/10.3390/cells10051194

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