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Cells
Volume 10
Issue 5
10.3390/cells10051127
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Article

A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells

by
Alessandro Cutarelli
1,
Vladimir A. Martínez-Rojas
2,†,
Alice Tata
1,†,
Ingrid Battistella
1,
Daniela Rossi
3,
Daniele Arosio
2,
Carlo Musio
2 and
Luciano Conti
1,*
1
Laboratory of Stem Cell Biology, Department of Cellular, Computational and Integrative Biology-CIBIO, University of Trento, 38123 Trento, Italy
2
Institute of Biophysics (IBF), Trento Unit, National Research Council (CNR) & LabSSAH, Bruno Kessler Foundation (FBK), 38123 Trento, Italy
3
Laboratory for Research on Neurodegenerative Disorders, Istituti Clinici Scientifici Maugeri IRCCS, 27100 Pavia, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Academic Editor: Andrea Pession
Cells 2021, 10(5), 1127; https://doi.org/10.3390/cells10051127
Received: 31 March 2021 / Revised: 27 April 2021 / Accepted: 4 May 2021 / Published: 7 May 2021
(This article belongs to the Special Issue Induced Pluripotent Stem Cells (IPSC) as a Disease Modeling and Drug Development Platform)
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Abstract

Methods for the conversion of human induced pluripotent stem cells (hiPSCs) into motor neurons (MNs) have opened to the generation of patient-derived in vitro systems that can be exploited for MN disease modelling. However, the lack of simplified and consistent protocols and the fact that hiPSC-derived MNs are often functionally immature yet limit the opportunity to fully take advantage of this technology, especially in research aimed at revealing the disease phenotypes that are manifested in functionally mature cells. In this study, we present a robust, optimized monolayer procedure to rapidly convert hiPSCs into enriched populations of motor neuron progenitor cells (MNPCs) that can be further amplified to produce a large number of cells to cover many experimental needs. These MNPCs can be efficiently differentiated towards mature MNs exhibiting functional electrical and pharmacological neuronal properties. Finally, we report that MN cultures can be long-term maintained, thus offering the opportunity to study degenerative phenomena associated with pathologies involving MNs and their functional, networked activity. These results indicate that our optimized procedure enables the efficient and robust generation of large quantities of MNPCs and functional MNs, providing a valid tool for MNs disease modelling and for drug discovery applications. View Full-Text
Keywords: induced pluripotent stem cells; hiPSC; spinal motor neurons; motor neuron progenitors; cellular models; spinal muscular atrophy induced pluripotent stem cells; hiPSC; spinal motor neurons; motor neuron progenitors; cellular models; spinal muscular atrophy
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MDPI and ACS Style

Cutarelli, A.; Martínez-Rojas, V.A.; Tata, A.; Battistella, I.; Rossi, D.; Arosio, D.; Musio, C.; Conti, L. A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells. Cells 2021, 10, 1127. https://doi.org/10.3390/cells10051127

AMA Style

Cutarelli A, Martínez-Rojas VA, Tata A, Battistella I, Rossi D, Arosio D, Musio C, Conti L. A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells. Cells. 2021; 10(5):1127. https://doi.org/10.3390/cells10051127

Chicago/Turabian Style

Cutarelli, Alessandro, Vladimir A. Martínez-Rojas, Alice Tata, Ingrid Battistella, Daniela Rossi, Daniele Arosio, Carlo Musio, and Luciano Conti. 2021. "A Monolayer System for the Efficient Generation of Motor Neuron Progenitors and Functional Motor Neurons from Human Pluripotent Stem Cells" Cells 10, no. 5: 1127. https://doi.org/10.3390/cells10051127

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