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Influence of Hypothermic Storage Fluids on Mesenchymal Stem Cell Stability: A Comprehensive Review and Personal Experience

1
Department of Histology and Embryology, Medical University of Warsaw, 02-004 Warsaw, Poland
2
Laboratory of Experimental Immunology, Military Institute of Hygiene and Epidemiology, 01-163 Warsaw, Poland
3
Department of Plastic Surgery, Medical Centre for Postgraduate Education, 00-416 Warsaw, Poland
4
Laboratory for Cell Research and Application, Medical University of Warsaw, 02-097 Warsaw, Poland
*
Author to whom correspondence should be addressed.
Academic Editors: Alessandro Poggi, Martin J. Hoogduijn and Marc-H. Dahlke
Cells 2021, 10(5), 1043; https://doi.org/10.3390/cells10051043
Received: 22 March 2021 / Revised: 25 April 2021 / Accepted: 26 April 2021 / Published: 28 April 2021
(This article belongs to the Special Issue Mesenchymal Stromal Cell‐Based Therapy)
Mesenchymal stem cells have generated a great deal of interest due to their potential use in regenerative medicine and tissue engineering. Examples illustrating their therapeutic value across various in vivo models are demonstrated in the literature. However, some clinical trials have not proved their therapeutic efficacy, showing that translation into clinical practice is considerably more difficult and discrepancies in clinical protocols can be a source of failure. Among the critical factors which play an important role in MSCs’ therapeutic efficiency are the method of preservation of the stem cell viability and various characteristics during their storage and transportation from the GMP production facility to the patient’s bedside. The cell storage medium should be considered a key factor stabilizing the environment and greatly influencing cell viability and potency and therefore the effectiveness of advanced therapy medicinal product (ATMP) based on MSCs. In this review, we summarize data from 826 publications concerning the effect of the most frequently used cell preservation solutions on MSC potential as cell-based therapeutic medicinal products. View Full-Text
Keywords: mesenchymal stem cells; cell therapy; preservation solutions; storage media; cell viability; quality control mesenchymal stem cells; cell therapy; preservation solutions; storage media; cell viability; quality control
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MDPI and ACS Style

Ścieżyńska, A.; Soszyńska, M.; Szpak, P.; Krześniak, N.; Malejczyk, J.; Kalaszczyńska, I. Influence of Hypothermic Storage Fluids on Mesenchymal Stem Cell Stability: A Comprehensive Review and Personal Experience. Cells 2021, 10, 1043. https://doi.org/10.3390/cells10051043

AMA Style

Ścieżyńska A, Soszyńska M, Szpak P, Krześniak N, Malejczyk J, Kalaszczyńska I. Influence of Hypothermic Storage Fluids on Mesenchymal Stem Cell Stability: A Comprehensive Review and Personal Experience. Cells. 2021; 10(5):1043. https://doi.org/10.3390/cells10051043

Chicago/Turabian Style

Ścieżyńska, Aneta, Marta Soszyńska, Patrycja Szpak, Natalia Krześniak, Jacek Malejczyk, and Ilona Kalaszczyńska. 2021. "Influence of Hypothermic Storage Fluids on Mesenchymal Stem Cell Stability: A Comprehensive Review and Personal Experience" Cells 10, no. 5: 1043. https://doi.org/10.3390/cells10051043

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