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Review

Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells

Institute for Cell and Tissue Culture Technology, Department of Biotechnology, University of Natural Resources and Life Sciences, Muthgasse 18, 1190 Vienna, Austria
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Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Academic Editor: Mehdi Najar
Cells 2021, 10(4), 886; https://doi.org/10.3390/cells10040886
Received: 23 March 2021 / Revised: 9 April 2021 / Accepted: 12 April 2021 / Published: 13 April 2021
(This article belongs to the Special Issue Feature Papers in Stem Cells)
Mesenchymal stem cells (MSCs) are of great interest for their use in cell-based therapies due to their multipotent differentiation and immunomodulatory capacities. In consequence of limited numbers following their isolation from the donor tissue, MSCs require extensive expansion performed in traditional 2D cell culture setups to reach adequate amounts for therapeutic use. However, prolonged culture of MSCs in vitro has been shown to decrease their differentiation potential and alter their immunomodulatory properties. For that reason, preservation of these physiological characteristics of MSCs throughout their in vitro culture is essential for improving the efficiency of therapeutic and in vitro modeling applications. With this objective in mind, many studies already investigated certain parameters for enhancing current standard MSC culture protocols with regard to the effects of specific culture media components or culture conditions. Although there is a lot of diversity in the final therapeutic uses of the cells, the primary stage of standard isolation and expansion is imperative. Therefore, we want to review on approaches for optimizing standard MSC culture protocols during this essential primary step of in vitro expansion. The reviewed studies investigate and suggest improvements focused on culture media components (amino acids, ascorbic acid, glucose level, growth factors, lipids, platelet lysate, trace elements, serum, and xenogeneic components) as well as culture conditions and processes (hypoxia, cell seeding, and dissociation during passaging), in order to preserve the MSC phenotype and functionality during the primary phase of in vitro culture. View Full-Text
Keywords: mesenchymal stem cells; physiologic cultivation; optimization cultivation mesenchymal stem cells; physiologic cultivation; optimization cultivation
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MDPI and ACS Style

Nikolits, I.; Nebel, S.; Egger, D.; Kreß, S.; Kasper, C. Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells. Cells 2021, 10, 886. https://doi.org/10.3390/cells10040886

AMA Style

Nikolits I, Nebel S, Egger D, Kreß S, Kasper C. Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells. Cells. 2021; 10(4):886. https://doi.org/10.3390/cells10040886

Chicago/Turabian Style

Nikolits, Ilias, Sabrina Nebel, Dominik Egger, Sebastian Kreß, and Cornelia Kasper. 2021. "Towards Physiologic Culture Approaches to Improve Standard Cultivation of Mesenchymal Stem Cells" Cells 10, no. 4: 886. https://doi.org/10.3390/cells10040886

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