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Article

The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection

1
Institute of Medical Microbiology and Hygiene, University of Saarland, D-66421 Homburg, Germany
2
Department of Pathology, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44511, Egypt
3
Laboratory of Pathogen Host Interactions, Université de Montpellier, CNRS, UMR 5235, 34095 Montpellier, France
4
Medical Microbiology and Immunology Department, Faculty of Medicine, Mansoura University, Mansoura 35516, Egypt
*
Authors to whom correspondence should be addressed.
Academic Editor: Alessandro Poggi
Cells 2021, 10(3), 645; https://doi.org/10.3390/cells10030645
Received: 16 February 2021 / Revised: 11 March 2021 / Accepted: 12 March 2021 / Published: 14 March 2021
(This article belongs to the Collection Feature Papers in Cellular Immunology)
Staphylococcus aureus continues to be a public health threat, especially in hospital settings. Studies aimed at deciphering the molecular and cellular mechanisms that underlie pathogenesis, host adaptation, and virulence are required to develop effective treatment strategies. Numerous host-pathogen interactions were found to be dependent on phosphatases-mediated regulation. This study focused on the analysis of the role of the low-molecular weight phosphatase PtpB, in particular, during infection. Deletion of ptpB in S. aureus strain SA564 significantly reduced the capacity of the mutant to withstand intracellular killing by THP-1 macrophages. When injected into normoglycemic C57BL/6 mice, the SA564 ΔptpB mutant displayed markedly reduced bacterial loads in liver and kidney tissues in a murine S. aureus abscess model when compared to the wild type. We also observed that PtpB phosphatase-activity was sensitive to oxidative stress. Our quantitative transcript analyses revealed that PtpB affects the transcription of various genes involved in oxidative stress adaptation and infectivity. Thus, this study disclosed first insights into the physiological role of PtpB during host interaction allowing us to link phosphatase-dependent regulation to oxidative bacterial stress adaptation during infection. View Full-Text
Keywords: Staphylococcus aureus; arginine phosphatase; infection; oxidative response Staphylococcus aureus; arginine phosphatase; infection; oxidative response
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MDPI and ACS Style

Elhawy, M.I.; Huc-Brandt, S.; Pätzold, L.; Gannoun-Zaki, L.; Abdrabou, A.M.M.; Bischoff, M.; Molle, V. The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection. Cells 2021, 10, 645. https://doi.org/10.3390/cells10030645

AMA Style

Elhawy MI, Huc-Brandt S, Pätzold L, Gannoun-Zaki L, Abdrabou AMM, Bischoff M, Molle V. The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection. Cells. 2021; 10(3):645. https://doi.org/10.3390/cells10030645

Chicago/Turabian Style

Elhawy, Mohamed I., Sylvaine Huc-Brandt, Linda Pätzold, Laila Gannoun-Zaki, Ahmed M.M. Abdrabou, Markus Bischoff, and Virginie Molle. 2021. "The Phosphoarginine Phosphatase PtpB from Staphylococcus aureus Is Involved in Bacterial Stress Adaptation during Infection" Cells 10, no. 3: 645. https://doi.org/10.3390/cells10030645

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