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Open AccessArticle

Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces

1
UGC de Reumatología, Hospital Clínico San Carlos, IdISSC, 28040 Madrid, Spain
2
UGC de Traumatología, Hospital Clínico San Carlos, IdISSC, 28040 Madrid, Spain
3
Servicio de Cirugía Oral y Maxilofacial, Hospital Clínico San Carlos, IdISSC, 28040 Madrid, Spain
4
Servicio de Cirugía I, Hospital Clínico San Carlos, IdISSC, 28040 Madrid, Spain
*
Author to whom correspondence should be addressed.
Co-senior authors.
Academic Editor: Marco Tatullo
Cells 2021, 10(2), 481; https://doi.org/10.3390/cells10020481
Received: 15 January 2021 / Revised: 17 February 2021 / Accepted: 19 February 2021 / Published: 23 February 2021
(This article belongs to the Special Issue Oral Stem Cells in Tissue Engineering and Regenerative Medicine)
Current gold-standard strategies for bone regeneration do not achieve the optimal recovery of bone biomechanical properties. To bypass these limitations, tissue engineering techniques based on hybrid materials made up of osteoprogenitor cells—such as mesenchymal stem cells (MSCs)—and bioactive ceramic scaffolds—such as calcium phosphate-based (CaPs) bioceramics—seem promising. The biological properties of MSCs are influenced by the tissue source. This study aims to define the optimal MSC source and construct (i.e., the MSC–CaP combination) for clinical application in bone regeneration. A previous iTRAQ analysis generated the hypothesis that anatomical proximity to bone has a direct effect on MSC phenotype. MSCs were isolated from adipose tissue, bone marrow, and dental pulp, then cultured both on a plastic surface and on CaPs (hydroxyapatite and β-tricalcium phosphate), to compare their biological features. On plastic, MSCs isolated from dental pulp (DPSCs) presented the highest proliferation capacity and the greatest osteogenic potential. On both CaPs, DPSCs demonstrated the greatest capacity to colonise the bioceramics. Furthermore, the results demonstrated a trend that DPSCs had the most robust increase in ALP activity. Regarding CaPs, β-tricalcium phosphate obtained the best viability results, while hydroxyapatite had the highest ALP activity values. Therefore, we propose DPSCs as suitable MSCs for cell-based bone regeneration strategies. View Full-Text
Keywords: mesenchymal stem cell; dental pulp; beta-tricalcium phosphate; hydroxyapatite; osteogenesis; regenerative medicine mesenchymal stem cell; dental pulp; beta-tricalcium phosphate; hydroxyapatite; osteogenesis; regenerative medicine
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MDPI and ACS Style

Mucientes, A.; Herranz, E.; Moro, E.; González-Corchón, A.; Peña-Soria, M.J.; Abasolo, L.; Rodriguez-Rodriguez, L.; Lamas, J.R.; Fernández-Gutiérrez, B. Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces. Cells 2021, 10, 481. https://doi.org/10.3390/cells10020481

AMA Style

Mucientes A, Herranz E, Moro E, González-Corchón A, Peña-Soria MJ, Abasolo L, Rodriguez-Rodriguez L, Lamas JR, Fernández-Gutiérrez B. Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces. Cells. 2021; 10(2):481. https://doi.org/10.3390/cells10020481

Chicago/Turabian Style

Mucientes, Arkaitz; Herranz, Eva; Moro, Enrique; González-Corchón, Aranzazu; Peña-Soria, María J.; Abasolo, Lydia; Rodriguez-Rodriguez, Luis; Lamas, Jose R.; Fernández-Gutiérrez, Benjamín. 2021. "Influence of Mesenchymal Stem Cell Sources on Their Regenerative Capacities on Different Surfaces" Cells 10, no. 2: 481. https://doi.org/10.3390/cells10020481

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