Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Vectors and Cell Transduction
2.2. Cell Culture and Differentiation
2.3. Cell Proliferation Measurement
2.4. Sample Preparation for Quantitative Proteomics
2.5. Sample Preparation for N-Terminomics
2.6. LC-MS/MS
2.7. Proteomic Data Analysis
2.8. Western Blot
2.9. Protein Glycation
3. Results
3.1. In Vitro System of Differentiated SH SY5Y Neuron-Like Cells
3.2. Degradome Analysis
3.3. Systemic Impact of DJ-1 ΔH8 on the Cellular Proteome
3.4. Protective Effect of DJ-1 on Protein Glycation
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Kern, U.; Fröhlich, K.; Bedacht, J.; Schmidt, N.; Biniossek, M.L.; Gensch, N.; Baerenfaller, K.; Schilling, O. Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells. Cells 2021, 10, 404. https://doi.org/10.3390/cells10020404
Kern U, Fröhlich K, Bedacht J, Schmidt N, Biniossek ML, Gensch N, Baerenfaller K, Schilling O. Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells. Cells. 2021; 10(2):404. https://doi.org/10.3390/cells10020404
Chicago/Turabian StyleKern, Ursula, Klemens Fröhlich, Johanna Bedacht, Nico Schmidt, Martin L. Biniossek, Nicole Gensch, Katja Baerenfaller, and Oliver Schilling. 2021. "Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells" Cells 10, no. 2: 404. https://doi.org/10.3390/cells10020404
APA StyleKern, U., Fröhlich, K., Bedacht, J., Schmidt, N., Biniossek, M. L., Gensch, N., Baerenfaller, K., & Schilling, O. (2021). Impact of DJ-1 and Helix 8 on the Proteome and Degradome of Neuron-Like Cells. Cells, 10(2), 404. https://doi.org/10.3390/cells10020404