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Article

The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction

1
Institute of Biophysics, Academy of Sciences of the Czech Republic, Královopolská 135, 612 65 Brno, Czech Republic
2
Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic
3
1st Faculty of Medicine, Charles University, Albertov 4, 128 00 Praha, Czech Republic
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Dechat
Cells 2021, 10(2), 297; https://doi.org/10.3390/cells10020297
Received: 2 November 2020 / Revised: 22 January 2021 / Accepted: 28 January 2021 / Published: 1 February 2021
(This article belongs to the Collection Lamins and Laminopathies)
The essential components of splicing are the splicing factors accumulated in nuclear speckles; thus, we studied how DNA damaging agents and A-type lamin depletion affect the properties of these regions, positive on the SC-35 protein. We observed that inhibitor of PARP (poly (ADP-ribose) polymerase), and more pronouncedly inhibitors of RNA polymerases, caused DNA damage and increased the SC-35 protein level. Interestingly, nuclear blebs, induced by PARP inhibitor and observed in A-type lamin-depleted or senescent cells, were positive on both the SC-35 protein and another component of the spliceosome, SRRM2. In the interphase cell nuclei, SC-35 interacted with the phosphorylated form of RNAP II, which was A-type lamin-dependent. In mitotic cells, especially in telophase, the SC-35 protein formed a well-visible ring in the cytoplasmic fraction and colocalized with β-catenin, associated with the plasma membrane. The antibody against the SRRM2 protein showed that nuclear speckles are already established in the cytoplasm of the late telophase and at the stage of early cytokinesis. In addition, we observed the occurrence of splicing factors in the nuclear blebs and micronuclei, which are also sites of both transcription and splicing. This conclusion supports the fact that splicing proceeds transcriptionally. According to our data, this process is A-type lamin-dependent. Lamin depletion also reduces the interaction between SC-35 and β-catenin in mitotic cells. View Full-Text
Keywords: splicing; SC-35; PARP inhibitor; RNA pol II splicing; SC-35; PARP inhibitor; RNA pol II
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MDPI and ACS Style

Legartová, S.; Fagherazzi, P.; Stixová, L.; Kovařík, A.; Raška, I.; Bártová, E. The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction. Cells 2021, 10, 297. https://doi.org/10.3390/cells10020297

AMA Style

Legartová S, Fagherazzi P, Stixová L, Kovařík A, Raška I, Bártová E. The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction. Cells. 2021; 10(2):297. https://doi.org/10.3390/cells10020297

Chicago/Turabian Style

Legartová, Soňa, Paolo Fagherazzi, Lenka Stixová, Aleš Kovařík, Ivan Raška, and Eva Bártová. 2021. "The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction" Cells 10, no. 2: 297. https://doi.org/10.3390/cells10020297

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