Next Article in Journal
Targeting Aberrant FGFR Signaling to Overcome CDK4/6 Inhibitor Resistance in Breast Cancer
Previous Article in Journal
The SC-35 Splicing Factor Interacts with RNA Pol II and A-Type Lamin Depletion Weakens This Interaction
 
 
Article

Mechanical Stress Induce PG-E2 in Murine Synovial Fibroblasts Originating from the Temporomandibular Joint

1
Department of Orthodontics, University Medical Centre of Regensburg, D-93053 Regensburg, Germany
2
Centre for Medical Biotechnology, Department of Orthopaedic Surgery, Experimental Orthopaedics, University of Regensburg, D-93053 Regensburg, Germany
3
Institute of Clinical Microbiology and Hygiene, University Hospital of Regensburg, D-93053 Regensburg, Germany
*
Author to whom correspondence should be addressed.
Contributed equally.
Academic Editor: Gundula Schulze-Tanzil
Cells 2021, 10(2), 298; https://doi.org/10.3390/cells10020298
Received: 11 January 2021 / Revised: 25 January 2021 / Accepted: 27 January 2021 / Published: 1 February 2021
Genetic predisposition, traumatic events, or excessive mechanical exposure provoke arthritic changes in the temporomandibular joint (TMJ). We analysed the impact of mechanical stress that might be involved in the development and progression of TMJ osteoarthritis (OA) on murine synovial fibroblasts (SFs) of temporomandibular origin. SFs were subjected to different protocols of mechanical stress, either to a high-frequency tensile strain for 4 h or to a tensile strain of varying magnitude for 48 h. The TMJ OA induction was evaluated based on the gene and protein secretion of inflammatory factors (Icam-1, Cxcl-1, Cxcl-2, Il-1ß, Il-1ra, Il-6, Ptgs-2, PG-E2), subchondral bone remodelling (Rankl, Opg), and extracellular matrix components (Col1a2, Has-1, collagen and hyaluronic acid deposition) using RT-qPCR, ELISA, and HPLC. A short high-frequency tensile strain had only minor effects on inflammatory factors and no effects on the subchondral bone remodelling induction or matrix constituent production. A prolonged tensile strain of moderate and advanced magnitude increased the expression of inflammatory factors. An advanced tensile strain enhanced the Ptgs-2 and PG-E2 expression, while the expression of further inflammatory factors were decreased. The tensile strain protocols had no effects on the RANKL/OPG expression, while the advanced tensile strain significantly reduced the deposition of matrix constituent contents of collagen and hyaluronic acid. The data indicates that the application of prolonged advanced mechanical stress on SFs promote PG-E2 protein secretion, while the deposition of extracellular matrix components is decreased. View Full-Text
Keywords: temporomandibular joint; osteoarthritis; synovitis; inflammation; mechanical strain temporomandibular joint; osteoarthritis; synovitis; inflammation; mechanical strain
Show Figures

Figure 1

MDPI and ACS Style

Nazet, U.; Feulner, L.; Muschter, D.; Neubert, P.; Schatz, V.; Grässel, S.; Jantsch, J.; Proff, P.; Schröder, A.; Kirschneck, C. Mechanical Stress Induce PG-E2 in Murine Synovial Fibroblasts Originating from the Temporomandibular Joint. Cells 2021, 10, 298. https://doi.org/10.3390/cells10020298

AMA Style

Nazet U, Feulner L, Muschter D, Neubert P, Schatz V, Grässel S, Jantsch J, Proff P, Schröder A, Kirschneck C. Mechanical Stress Induce PG-E2 in Murine Synovial Fibroblasts Originating from the Temporomandibular Joint. Cells. 2021; 10(2):298. https://doi.org/10.3390/cells10020298

Chicago/Turabian Style

Nazet, Ute, Laura Feulner, Dominique Muschter, Patrick Neubert, Valentin Schatz, Susanne Grässel, Jonathan Jantsch, Peter Proff, Agnes Schröder, and Christian Kirschneck. 2021. "Mechanical Stress Induce PG-E2 in Murine Synovial Fibroblasts Originating from the Temporomandibular Joint" Cells 10, no. 2: 298. https://doi.org/10.3390/cells10020298

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop