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Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM)

1
Departamento de Biotecnología, Universidad de Alicante, 03690 Alicante, Spain
2
Cátedra Human Fertility, Universidad de Alicante, 03080 Alicante, Spain
3
IVF Spain, Medicina Reproductiva, 03540 Alicante, Spain
4
Departamento de Biología Celular e Histología, Universidad de Murcia, Instituto Murciano de Investigación Sociosanitaria (IMIB), 30003 Murcia, Spain
*
Author to whom correspondence should be addressed.
These authors contributed equally to this work.
Cells 2021, 10(2), 222; https://doi.org/10.3390/cells10020222
Received: 2 January 2021 / Revised: 16 January 2021 / Accepted: 21 January 2021 / Published: 23 January 2021
(This article belongs to the Special Issue Molecular Physiology of Human Sperm Cells)
Capacitation drives sperm biophysical and biochemical changes for sperm-oocyte interactions. It is a well-known fact that the molecular complex arylsulfatase A (ARSA), hyaluronidase sperm adhesion molecule 1 (SPAM1), and heat shock protein 2 (HSPA2) plays a significant role in sperm–zona pellucida (ZP) binding. However, the time-dependent capacitation effects on the sperm surface ARSA presence and specific topographic distributions remain to be elucidated. Here, we quantified the ARSA density and specific membrane domain locations before (US) and after in vitro capacitation (one and four hours; CS1–CS4) in human sperm using high-resolution field emission scanning electron microscopy (FE-SEM) and immunogold labeling. Our results showed a significant and progressive capacitation-mediated increase of labeled spermatozoa from the US (37%) to CS4 (100%) physiological conditions. In addition, surface mapping revealed a close relationship between the ARSA residues and their acrosomal repositioning. Compared with the ARSA surface heterogeneous distribution found in US, the CS1–4 conditions exhibited clustering on the peri-acrosomal region, showing that time-dependent capacitation also induced a ARSA residue dramatic translocation on sperm surfaces. Our findings provide novel insights into the molecular remodeling events preceding sperm-oocyte interactions. View Full-Text
Keywords: spermatozoa; capacitation; ARSA; FE-SEM; gold-labeling spermatozoa; capacitation; ARSA; FE-SEM; gold-labeling
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MDPI and ACS Style

Gómez-Torres, M.J.; Huerta-Retamal, N.; Robles-Gómez, L.; Sáez-Espinosa, P.; Aizpurua, J.; Avilés, M.; Romero, A. Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM). Cells 2021, 10, 222. https://doi.org/10.3390/cells10020222

AMA Style

Gómez-Torres MJ, Huerta-Retamal N, Robles-Gómez L, Sáez-Espinosa P, Aizpurua J, Avilés M, Romero A. Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM). Cells. 2021; 10(2):222. https://doi.org/10.3390/cells10020222

Chicago/Turabian Style

Gómez-Torres, María J.; Huerta-Retamal, Natalia; Robles-Gómez, Laura; Sáez-Espinosa, Paula; Aizpurua, Jon; Avilés, Manuel; Romero, Alejandro. 2021. "Arylsulfatase A Remodeling during Human Sperm In Vitro Capacitation Using Field Emission Scanning Electron Microscopy (FE-SEM)" Cells 10, no. 2: 222. https://doi.org/10.3390/cells10020222

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