Next Article in Journal
Methods for Studying Endometrial Pathology and the Potential of Atomic Force Microscopy in the Research of Endometrium
Next Article in Special Issue
Chemically Defined Xeno- and Serum-Free Cell Culture Medium to Grow Human Adipose Stem Cells
Previous Article in Journal
Nurse-Like Cells and Chronic Lymphocytic Leukemia B Cells: A Mutualistic Crosstalk inside Tissue Microenvironments
Previous Article in Special Issue
Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization
Open AccessArticle

Multiplex Analysis of Adipose-Derived Stem Cell (ASC) Immunophenotype Adaption to In Vitro Expansion

Regenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Fredrik Bajers Vej 3B, 9220 Aalborg, Denmark
Author to whom correspondence should be addressed.
Academic Editor: Christian Dani
Cells 2021, 10(2), 218;
Received: 17 December 2020 / Revised: 11 January 2021 / Accepted: 19 January 2021 / Published: 22 January 2021
(This article belongs to the Special Issue Human Adipose Stem Cells)
In order to enhance the therapeutic potential, it is important that sufficient knowledge regarding the dynamic changes of adipose-derived stem cell (ASC) immunophenotypical and biological properties during in vitro growth is available. Consequently, we embarked on a study to follow the evolution of highly defined cell subsets from three unrelated donors in the course of eight passages on tissue culture polystyrene. The co-expression patterns were defined by panels encompassing seven and five cell surface markers, including CD34, CD146, CD166, CD200, CD248, CD271, and CD274 and CD29, CD31, CD36, CD201, and Stro-1, respectively. The analysis was performed using multichromatic flow cytometry. We observed a major paradigm shift, where the CD166-CD34+ combination which was found across all cell subsets early in the culture was replaced by the CD166+ phenotype as the population homogeneity increased with time. At all analysis points, the cultures were dominated by a few major clones that were highly prevalent in most of the donors. The selection process resulted in two predominant clones in the larger panel (CD166+CD34CD146CD271 CD274CD248CD200 and CD166+CD34+ CD146CD271CD274CD248CD200) and one clone in the smaller panel (CD29+CD201+CD36 Stro-1 CD31). The minor subsets, including CD166+CD34CD146CD271+CD274CD248CD200 and CD166+CD34+CD146+CD271CD274CD248CD200, and CD29+CD201CD36Stro-1CD31, CD29+CD201+CD36Stro-1+CD31, and CD29+CD201+CD36+Stro-1CD31, in the seven and five marker panels, respectively, were, on the other, hand highly fluctuating and donor-dependent. The results demonstrate that only a limited number of phenotypical repertoires are possible in ASC cultures. Marked differences in their relative occurrence between distinct individuals underscore the need for potency standardization of different ASC preparation to improve the clinical outcome. View Full-Text
Keywords: adipose-derived stem cells; heterogeneity; immunophenotype; cell subsets adipose-derived stem cells; heterogeneity; immunophenotype; cell subsets
Show Figures

Figure 1

MDPI and ACS Style

Peng, Q.; Duda, M.; Ren, G.; Xuan, Z.; Pennisi, C.P.; Porsborg, S.R.; Fink, T.; Zachar, V. Multiplex Analysis of Adipose-Derived Stem Cell (ASC) Immunophenotype Adaption to In Vitro Expansion. Cells 2021, 10, 218.

AMA Style

Peng Q, Duda M, Ren G, Xuan Z, Pennisi CP, Porsborg SR, Fink T, Zachar V. Multiplex Analysis of Adipose-Derived Stem Cell (ASC) Immunophenotype Adaption to In Vitro Expansion. Cells. 2021; 10(2):218.

Chicago/Turabian Style

Peng, Qiuyue; Duda, Martyna; Ren, Guoqiang; Xuan, Zongzhe; Pennisi, Cristian P.; Porsborg, Simone R.; Fink, Trine; Zachar, Vladimir. 2021. "Multiplex Analysis of Adipose-Derived Stem Cell (ASC) Immunophenotype Adaption to In Vitro Expansion" Cells 10, no. 2: 218.

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

Search more from Scilit
Back to TopTop