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Article

Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells

1
Department of Morphology and Cell Biology, Faculty of Medicine, University of Oviedo, Julián Clavería s/n, 33006 Oviedo, Spain
2
Instituto de Investigación Sanitaria del Principado de Asturias (ISPA), 33011 Oviedo, Spain
3
Instituto de Neurociencias del Principado de Asturias (INEUROPA), 33006 Oviedo, Spain
4
CNC-Center for Neuroscience and Cell Biology, University of Coimbra, 3060-197 Cantanhede, Portugal
*
Author to whom correspondence should be addressed.
Academic Editor: Leo Veenman
Cells 2021, 10(10), 2784; https://doi.org/10.3390/cells10102784
Received: 22 July 2021 / Revised: 29 September 2021 / Accepted: 5 October 2021 / Published: 17 October 2021
(This article belongs to the Special Issue Advances in translocator protein (TSPO) research)
The 18-kDa translocator protein (TSPO) is a key mitochondrial target by which different TSPO ligands exert neuroprotective effects. We assayed the neurogenic potential of TSPO to induce the neuronal differentiation of pluripotent P19 stem cells in vitro. We studied changes in cell morphology, cell proliferation, cell death, the cell cycle, mitochondrial functionality, and the levels of pluripotency and neurogenesis of P19 stem cells treated with the TSPO ligand, PK 11195, in comparison to differentiation induced by retinoid acid (RA) and undifferentiated P19 stem cells. We observed that PK 11195 was able to activate the differentiation of P19 stem cells by promoting the development of embryoid bodies. PK 11195 also induced changes in the cell cycle, decreased cell proliferation, and activated cell death. Mitochondrial metabolism was also enhanced by PK 11195, thus increasing the levels of reactive oxygen species, Ca2+, and ATP as well as the mitochondrial membrane potential. Markers of pluripotency and neurogenesis were also altered during the cell differentiation process, as PK 11195 induced the differentiation of P19 stem cells with a high predisposition toward a neuronal linage, compared to cell differentiation induced by RA. Thus, we suggest a relevant neurogenic potential of TSPO along with broad therapeutic implications. View Full-Text
Keywords: TSPO; mitochondria; stem cells; neurogenesis TSPO; mitochondria; stem cells; neurogenesis
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MDPI and ACS Style

González-Blanco, L.; Bermejo-Millo, J.C.; Oliveira, G.; Potes, Y.; Antuña, E.; Menéndez-Valle, I.; Vega-Naredo, I.; Coto-Montes, A.; Caballero, B. Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells. Cells 2021, 10, 2784. https://doi.org/10.3390/cells10102784

AMA Style

González-Blanco L, Bermejo-Millo JC, Oliveira G, Potes Y, Antuña E, Menéndez-Valle I, Vega-Naredo I, Coto-Montes A, Caballero B. Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells. Cells. 2021; 10(10):2784. https://doi.org/10.3390/cells10102784

Chicago/Turabian Style

González-Blanco, Laura, Juan C. Bermejo-Millo, Gabriela Oliveira, Yaiza Potes, Eduardo Antuña, Iván Menéndez-Valle, Ignacio Vega-Naredo, Ana Coto-Montes, and Beatriz Caballero. 2021. "Neurogenic Potential of the 18-kDa Mitochondrial Translocator Protein (TSPO) in Pluripotent P19 Stem Cells" Cells 10, no. 10: 2784. https://doi.org/10.3390/cells10102784

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