Next Article in Journal
siRNA Nanoparticle Targeting PD-L1 Activates Tumor Immunity and Abrogates Pancreatic Cancer Growth in Humanized Preclinical Model
Previous Article in Journal
Activation of mTORC1 by Free Fatty Acids Suppresses LAMP2 and Autophagy Function via ER Stress in Alcohol-Related Liver Disease
Previous Article in Special Issue
Impaired Bile Acid Metabolism and Gut Dysbiosis in Mice Lacking Lysosomal Acid Lipase
Article

Steatosis Alters the Activity of Hepatocyte Membrane Transporters in Obese Rats

1
Department of Radiology, University Hospital of Geneva, 1205 Geneva, Switzerland
2
Centre de Recherche sur L’inflammation, Inserm, U1149, CNRS, ERL8252, F-75006 Paris, France
3
Department of Radiology, Hôpital Beaujon, Université de Paris, 92110 Clichy, France
4
Department of Radiology, Hôpitaux Paris Nord Val de Seine (AP-HP), Université de Paris, 92110 Clichy, France
*
Author to whom correspondence should be addressed.
Academic Editor: Jérôme Eeckhoute
Cells 2021, 10(10), 2733; https://doi.org/10.3390/cells10102733
Received: 16 September 2021 / Revised: 4 October 2021 / Accepted: 5 October 2021 / Published: 13 October 2021
(This article belongs to the Special Issue Cellular and Molecular Mechanisms Governing Liver Pathophysiology)
Fat accumulation (steatosis) in ballooned hepatocytes alters the expression of membrane transporters in Zucker fatty (fa/fa) rats. The aim of the study was to quantify the functions of these transporters and their impact on hepatocyte concentrations using a clinical hepatobiliary contrast agent (Gadobenate dimeglumine, BOPTA) for liver imaging. In isolated and perfused rat livers, we quantified BOPTA accumulation and decay profiles in fa/+ (normal) and fa/fa hepatocytes by placing a gamma counter over livers. Profiles of BOPTA accumulation and decay in hepatocytes were analysed with nonlinear regressions to characterise BOPTA influx and efflux across hepatocyte transporters. At the end of the accumulation period, BOPTA hepatocyte concentrations and influx clearances were not significantly different in fa/+ and fa/fa livers. In contrast, bile clearance was significantly lower in fatty hepatocytes while efflux clearance back to sinusoids compensated the low efflux into canaliculi. The time when BOPTA cellular efflux impacts the accumulation profile of hepatocyte concentrations was slightly delayed (2 min) by steatosis, anticipating a delayed emptying of hepatocytes. The experimental model is useful for quantifying the functions of hepatocyte transporters in liver diseases. View Full-Text
Keywords: hepatocyte membrane transporter; hepatocyte concentrations; transporter activity; liver imaging hepatocyte membrane transporter; hepatocyte concentrations; transporter activity; liver imaging
Show Figures

Figure 1

MDPI and ACS Style

Pastor, C.M.; Vilgrain, V. Steatosis Alters the Activity of Hepatocyte Membrane Transporters in Obese Rats. Cells 2021, 10, 2733. https://doi.org/10.3390/cells10102733

AMA Style

Pastor CM, Vilgrain V. Steatosis Alters the Activity of Hepatocyte Membrane Transporters in Obese Rats. Cells. 2021; 10(10):2733. https://doi.org/10.3390/cells10102733

Chicago/Turabian Style

Pastor, Catherine M., and Valérie Vilgrain. 2021. "Steatosis Alters the Activity of Hepatocyte Membrane Transporters in Obese Rats" Cells 10, no. 10: 2733. https://doi.org/10.3390/cells10102733

Find Other Styles
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map by Country/Region

1
Back to TopTop