Exendin-4 Pretreatment Attenuates Kainic Acid-Induced Hippocampal Neuronal Death
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Ex-4-Albumin Binding Domain (ABD) Protein Expression and Purification
2.3. Drug Treatment and Seizure Mouse Model
2.4. Enzyme-Linked Immunosorbent Assay (ELISA)
2.5. Tissue Preparation and Cresyl Violet Staining
2.6. Terminal Deoxynucleotidyl Transferase dUTP Nick End Labeling (TUNEL) Assay
2.7. Western Blot Analysis
2.8. Proximity Ligation Assay (PLA)
2.9. Double Immunofluorescence
2.10. Immunohistochemistry
2.11. Quantification of Histological Data
2.12. Statistical Analysis
3. Results
3.1. KA Treatment Affects the Hippocampal Expression of Heme Oxygenase-1 (HO-1) and GLP-1R in Mice
3.2. Ex-4 Pretreatment Reduces KA-Induced Seizure Activity and Hippocampal Cell Death in Mice
3.3. Ex-4 Pretreatment Prevents the Inhibition of GLP-1R Protein Expression in the Hippocampus of KA-Treated Mice
3.4. Ex-4 Pretreatment Attenuates Neuroinflammation in KA-Treated Mice
3.5. Ex-4 Pretreatment Attenuates Neuroinflammation in KA-Treated Mice
3.6. Ex-4 Pretreatment Protects against BBB Leakage in KA-Treated Mouse Hippocampus
3.7. Ex-4 Pretreatment Affects Hippocampal p-CREB Expression in KA-Treated Mice
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Ahn, Y.-J.; Shin, H.-J.; Jeong, E.-A.; An, H.-S.; Lee, J.-Y.; Jang, H.-M.; Kim, K.-E.; Lee, J.; Shin, M.-C.; Roh, G.-S. Exendin-4 Pretreatment Attenuates Kainic Acid-Induced Hippocampal Neuronal Death. Cells 2021, 10, 2527. https://doi.org/10.3390/cells10102527
Ahn Y-J, Shin H-J, Jeong E-A, An H-S, Lee J-Y, Jang H-M, Kim K-E, Lee J, Shin M-C, Roh G-S. Exendin-4 Pretreatment Attenuates Kainic Acid-Induced Hippocampal Neuronal Death. Cells. 2021; 10(10):2527. https://doi.org/10.3390/cells10102527
Chicago/Turabian StyleAhn, Yu-Jeong, Hyun-Joo Shin, Eun-Ae Jeong, Hyeong-Seok An, Jong-Youl Lee, Hye-Min Jang, Kyung-Eun Kim, Jaewoong Lee, Meong-Cheol Shin, and Gu-Seob Roh. 2021. "Exendin-4 Pretreatment Attenuates Kainic Acid-Induced Hippocampal Neuronal Death" Cells 10, no. 10: 2527. https://doi.org/10.3390/cells10102527