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Article

Generation of Functional Vascular Endothelial Cells and Pericytes from Keratinocyte Derived Human Induced Pluripotent Stem Cells

1
Institute of Neuroanatomy & Developmental Biology (INDB), Eberhard Karls University Tübingen, Österbergstrasse 3, 72074 Tübingen, Germany
2
Department of Internal Medicine 1, Ulm University Hospital, 89081 Ulm, Germany
*
Author to whom correspondence should be addressed.
These authors contribute equal to this work.
Cells 2021, 10(1), 74; https://doi.org/10.3390/cells10010074
Received: 20 November 2020 / Revised: 18 December 2020 / Accepted: 30 December 2020 / Published: 5 January 2021
(This article belongs to the Special Issue Pluripotent Stem Cells for Regenerative Medicine)
Human induced pluripotent stem cell (hiPSC)-derived endothelial cells (ECs) and pericytes provide a powerful tool for cardiovascular disease modelling, personalized drug testing, translational medicine, and tissue engineering. Here, we report a novel differentiation protocol that results in the fast and efficient production of ECs and pericytes from keratinocyte-derived hiPSCs. We found that the implementation of a 3D embryoid body (EB) stage significantly improves the differentiation efficiency. Compared with the monolayer-based technique, our protocol yields a distinct EC population with higher levels of EC marker expression such as CD31 and vascular endothelial cadherin (VE-cadherin). Furthermore, the EB-based protocol allows the generation of functional EC and pericyte populations that can promote blood vessel-like structure formation upon co-culturing. Moreover, we demonstrate that the EB-based ECs and pericytes can be successfully used in a microfluidic chip model, forming a stable 3D microvascular network. Overall, the described protocol can be used to efficiently differentiate both ECs and pericytes with distinct and high marker expression from keratinocyte-derived hiPSCs, providing a potent source material for future cardiovascular disease studies. View Full-Text
Keywords: human pluripotent stem cells; hiPSC-derived endothelial cells; hiPSC-derived pericytes; vasculature-on-a-chip; self-assembled microvascular network human pluripotent stem cells; hiPSC-derived endothelial cells; hiPSC-derived pericytes; vasculature-on-a-chip; self-assembled microvascular network
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MDPI and ACS Style

Pars, S.; Achberger, K.; Kleger, A.; Liebau, S.; Pashkovskaia, N. Generation of Functional Vascular Endothelial Cells and Pericytes from Keratinocyte Derived Human Induced Pluripotent Stem Cells. Cells 2021, 10, 74. https://doi.org/10.3390/cells10010074

AMA Style

Pars S, Achberger K, Kleger A, Liebau S, Pashkovskaia N. Generation of Functional Vascular Endothelial Cells and Pericytes from Keratinocyte Derived Human Induced Pluripotent Stem Cells. Cells. 2021; 10(1):74. https://doi.org/10.3390/cells10010074

Chicago/Turabian Style

Pars, Selin, Kevin Achberger, Alexander Kleger, Stefan Liebau, and Natalia Pashkovskaia. 2021. "Generation of Functional Vascular Endothelial Cells and Pericytes from Keratinocyte Derived Human Induced Pluripotent Stem Cells" Cells 10, no. 1: 74. https://doi.org/10.3390/cells10010074

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