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Screening Tolerance to Phosphorus Deficiency and Validation of Phosphorus Uptake 1 (Pup1) Gene-Linked Markers in Thai Indigenous Upland Rice Germplasm

1
Department of Agronomy, Faculty of Agriculture, Khon Kaen University, Khon Kaen 40002, Thailand
2
Department of Agronomy, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen, Nakhon Pathom 73140, Thailand
3
Associate Fellow of the Royal Society of Thailand, Sanam Suea Pa, Dusit, Bangkok 10300, Thailand
*
Author to whom correspondence should be addressed.
Agronomy 2019, 9(2), 81; https://doi.org/10.3390/agronomy9020081
Received: 2 January 2019 / Revised: 28 January 2019 / Accepted: 11 February 2019 / Published: 12 February 2019
(This article belongs to the Special Issue Soil Phosphorus Dynamics: Agronomic and Environmental Impacts)
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Abstract

Phosphorus (P) deficiency is a major factor limiting rice yield throughout the world. Fortunately, some rice accessions are tolerant and can thrive well, even in soils with low P content. The ability to uptake P is heritable, and thus can be incorporated into rice cultivars through standard breeding methods. The objective of this study was to screen for tolerance to phosphorus deficiency and validate the tolerant accessions with phosphorus uptake 1 (Pup1) gene-linked markers in Thai indigenous upland rice germplasm. One hundred sixty-eight rice varieties were screened in a solution culture and assigned a phosphorus deficiency tolerance index and plant symptom score. Eleven upland rice accessions (ULR026, ULR031, ULR124, ULR145, ULR180, ULR183, ULR185, ULR186, ULR213, ULR260, and ULR305), together with the lowland rice cultivar (PLD), were classified as tolerant. They were each validated by nine markers linked to the Pup1 locus and observed for the expected polymerase chain reaction (PCR) product of 0 to 9 markers. The presence or absence of the tolerant allele at the Pup1 locus showed only a slight relationship with the tolerance. Moreover, some lines such as ULR183 and ULR213 expressed high tolerance without the Pup1-linked gene product. Both accessions are useful for the exploration of novel genes conferring tolerance to phosphorus deficiency. View Full-Text
Keywords: phosphorus deficiency tolerance index; harvesting index; root dry weight; Yoshida solution; plant symptom score phosphorus deficiency tolerance index; harvesting index; root dry weight; Yoshida solution; plant symptom score
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Chankaew, S.; Monkham, T.; Pinta, W.; Sanitchon, J.; Kaewpradit, W.; Srinives, P. Screening Tolerance to Phosphorus Deficiency and Validation of Phosphorus Uptake 1 (Pup1) Gene-Linked Markers in Thai Indigenous Upland Rice Germplasm. Agronomy 2019, 9, 81.

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