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Open AccessArticle

Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers

1
Department of Botany and Plant Pathology, Oregon State University, Corvallis, OR 97331, USA
2
Hermiston Agricultural Research and Extension Center, Oregon State University, Hermiston, OR 97838, USA
3
Tecnologico de Monterrey, Escuela de Ingeniería y Ciencias, Monterrey, Nuevo León, Mexico
4
USDA/Agricultural Research Service, Sturgeon Bay, WI 54235, USA
*
Author to whom correspondence should be addressed.
Agronomy 2019, 9(11), 734; https://doi.org/10.3390/agronomy9110734
Received: 30 September 2019 / Revised: 30 October 2019 / Accepted: 6 November 2019 / Published: 9 November 2019
(This article belongs to the Special Issue Biofortification of Crops)
Biofortification of folates in staple crops is an important strategy to help eradicate human folate deficiencies. Folate biofortification using genetic engineering has shown great success in rice grain, tomato fruit, lettuce, and potato tuber. However, consumers’ skepticism, juridical hurdles, and lack of economic model have prevented the widespread adoption of nutritionally-enhanced genetically-engineered (GE) food crops. Meanwhile, little effort has been made to biofortify food crops with folate by breeding. Previously we reported >10-fold variation in folate content in potato genotypes. To facilitate breeding for enhanced folate content, we attempted to identify genes that control folate content in potato tuber. For this, we analyzed the expression of folate biosynthesis and salvage genes in low- and high-folate potato genotypes. First, RNA-Seq analysis showed that, amongst all folate biosynthesis and salvage genes analyzed, only one gene, which encodes γ-glutamyl hydrolase 1 (GGH1), was consistently expressed at higher levels in high- compared to low-folate segregants of a Solanum boliviense Dunal accession. Second, quantitative PCR showed that GGH1 transcript levels were higher in high- compared to low-folate segregants for seven out of eight pairs of folate segregants analyzed. These results suggest that GGH1 gene expression is an indicator of folate content in potato tubers. View Full-Text
Keywords: folate; regulation; potato folate; regulation; potato
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Robinson, B.R.; Garcia Salinas, C.; Ramos Parra, P.; Bamberg, J.; Diaz de la Garza, R.I.; Goyer, A. Expression Levels of the γ-Glutamyl Hydrolase I Gene Predict Vitamin B9 Content in Potato Tubers. Agronomy 2019, 9, 734.

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