Genome-Wide Identification and Expression Analysis of Heat Shock Transcription Factor Family in Chenopodium quinoa Willd
State Key Laboratory of 1Crop Stress Biology in Arid Areas and College of Agronomy, Northwest A&F University, Yangling 712100, Shaanxi, China
Department of Plant Science, Xizang Agriculture and Animal Husbandry College, Linzhi 860000, Tibet, China
Authors to whom correspondence should be addressed.
These authors contributed equally to this work.
Agronomy 2018, 8(7), 103; https://doi.org/10.3390/agronomy8070103
Received: 1 May 2018 / Revised: 7 June 2018 / Accepted: 21 June 2018 / Published: 28 June 2018
Heat shock transcription factor (Hsf) is one of the conserved gene families in plants, playing a crucial role in growth and development, as well as in response to diverse stresses. Although it has been systematically studied in many species, little is known about the Hsf gene family in Chenopodium quinoa, especially those involved in the regulatory network of stress processes. In this study, we identified 23 Hsf genes in quinoa (CqHsfs) through a genome-wide search method based on the latest available genome information. Phylogenetic analysis classified them into three groups, and group A was further divided into nine subgroups, which was supported by conserved domain organizations. Gene structure and multiple sequence alignment analysis revealed that all of the CqHsfs possessed a similar structure organization and were highly conserved in BDB domain. Interaction network analysis identified 13 CqHsfs involved in the network pathway to regulate diverse biological processes. Expression profiles of these CqHsfs were further investigated using the RNA-seq data, and tissue-specific and stress-responsive candidates were identified. Finally, four heat-responsive CqHsfs were selected to validate their expression level through semi-quantitative RT-PCR analysis. This study reported the organization, structure, and expression profiles of the Hsf gene family in quinoa, which will contributes to further functional analysis, and helps to better understand the roles and regulatory mechanism of heat shock factors playing in quinoa and beyond.