Candidate Gene Identification for a Lethal Chlorophyll-Deficient Mutant in Soybean
Abstract
:1. Introduction
2. Results and Discussion
Population | Gene | No. F2 plants | No. F2:3 families | ||||||
---|---|---|---|---|---|---|---|---|---|
Green | Yellow | χ2 (3:1) | P | All Green | Segregating | χ2 (1:2) | P | ||
PR350-1 | YL_PR350 | 137 | 36 | 1.62 | 0.20 | 38 | 94 | 1.23 | 0.27 |
PR350-6 | YL_PR350 | 153 | 55 | 0.23 | 0.63 | 59 | 83 | 4.32 | 0.04 |
Total | 290 | 91 | 0.25 | 0.62 | 97 | 177 | 0.53 | 0.47 |
Gene | Start Position (bp) | End Position (bp) | Predicted Protein/Function |
---|---|---|---|
Glyma.15g275700 | 51472288 | 51473210 | No functional annotation |
Glyma.15g275800 | 51473359 | 51476124 | Unknown |
Glyma.15g275900 | 51486036 | 51491942 | Biogenesis of Photosystem I and II and chloroplast ultrastructure |
Glyma.15g276000 | 51495263 | 51502516 | DNA Gyrase/Topoisomerase IV, DNA binding, ATP binding |
Glyma.15g276100 | 51510448 | 51513137 | Unknown (DUF642) |
Glyma.15g276200 | 51533618 | 51534670 | Unknown |
Glyma.15g276300 | 51541078 | 51546842 | Sterol methyltransferase C-terminal, steroid biosynthetic process |
Glyma.15g276400 | 51549706 | 51555332 | Copper Amine oxidase |
Glyma.15g276500 | 51564250 | 51573306 | Flavin containing amine oxidoreductase |
Glyma.15g276600 | 51574389 | 51574676 | Wound induced protein |
Glyma.15g276700 | 51578950 | 51593999 | Uncharacterized protein family, UPF0114 |
Glyma.15g276800 | 51600225 | 51651653 | Mandelate racemase / muconate lactonizing enzyme, Thiamine pyrophosphate enzyme |
Glyma.15g275600 | 51456041 | 51457063 | Unknown |
3. Experimental Section
3.1. Plant Material
3.2. DNA Isolation and Bulked Segregant Analysis (BSA)
3.3. Molecular Analysis
4. Conclusions
Acknowledgements
Author Contribution
Conflict of Interest
References and Notes
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Reed, S.; Atkinson, T.; Gorecki, C.; Espinosa, K.; Przybylski, S.; Goggi, A.S.; Palmer, R.G.; Sandhu, D. Candidate Gene Identification for a Lethal Chlorophyll-Deficient Mutant in Soybean. Agronomy 2014, 4, 462-469. https://doi.org/10.3390/agronomy4040462
Reed S, Atkinson T, Gorecki C, Espinosa K, Przybylski S, Goggi AS, Palmer RG, Sandhu D. Candidate Gene Identification for a Lethal Chlorophyll-Deficient Mutant in Soybean. Agronomy. 2014; 4(4):462-469. https://doi.org/10.3390/agronomy4040462
Chicago/Turabian StyleReed, Sam, Taylor Atkinson, Carly Gorecki, Katherine Espinosa, Sarah Przybylski, Alcira Susana Goggi, Reid G. Palmer, and Devinder Sandhu. 2014. "Candidate Gene Identification for a Lethal Chlorophyll-Deficient Mutant in Soybean" Agronomy 4, no. 4: 462-469. https://doi.org/10.3390/agronomy4040462