Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.)

Kim, JangChol; An, Xin; Yang, Ke; Miao, Si; Qin, Yushi; Hu, Yinxia; Du, Bo; Zhu, Lili; He, Guangcun; Chen, Rongzhi

doi:10.3390/agronomy12040808

Open AccessArticle

Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.)

by

JangChol Kim

^1,2,†,

Xin An

^1,2,†,

Ke Yang

^1,2,

Si Miao

^1,2,

Yushi Qin

^1,2,

Yinxia Hu

^1,2,

Bo Du

¹,

Lili Zhu

¹,

Guangcun He

¹

and

Rongzhi Chen

^1,2,*

¹

State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University, Wuhan 430072, China

²

Wuhan University Shenzhen Research Institute, Shenzhen 518057, China

^*

Author to whom correspondence should be addressed.

^†

These authors contributed equally to this work.

Agronomy 2022, 12(4), 808; https://doi.org/10.3390/agronomy12040808

Submission received: 23 February 2022 / Revised: 20 March 2022 / Accepted: 26 March 2022 / Published: 27 March 2022

(This article belongs to the Special Issue A Themed Issue in Memory of Academician Zhu Yingguo (1939–2017))

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Abstract

Brown planthopper (BPH) has become the most devastating insect pests of rice and a serious threat to rice production. To combat newly occurring virulent BPH populations, it is still urgent to explore more new broad-spectrum BPH resistance genes and integrate them into rice cultivars. In the present study, we explored the genetic basis of BPH resistance in IRGC 8678. We identified and mapped a new resistance gene Bph43 to a region of ~380 kb on chromosome 11. Genes encoding nucleotide-binding domain leucine-rich repeat-containing (NBS-LRR)-type disease resistance proteins or Leucine Rich Repeat family proteins annotated in this region were predicted as the possible candidates for Bph43. Meanwhile, we developed near isogenic lines of Bph43 (NIL-Bph43-9311) in an elite restorer line 9311 background using marker-assisted selection (MAS). The further characterization of NIL-Bph43-9311 demonstrated that Bph43 confers strong antibiosis and antixenosis effects on BPH. Comparative transcriptome analysis revealed that genes related to the defense response and resistance gene-dependent signaling pathway were significantly and uniquely enriched in BPH-infested NIL-Bph43-9311. Our work demonstrated that Bph43 can be deployed as a valuable donor in BPH resistance breeding programs.

Keywords: brown planthopper; gene mapping; near isogenic line; marker-assisted selection; RNA-Seq

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MDPI and ACS Style

Kim, J.; An, X.; Yang, K.; Miao, S.; Qin, Y.; Hu, Y.; Du, B.; Zhu, L.; He, G.; Chen, R. Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.). Agronomy 2022, 12, 808. https://doi.org/10.3390/agronomy12040808

AMA Style

Kim J, An X, Yang K, Miao S, Qin Y, Hu Y, Du B, Zhu L, He G, Chen R. Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.). Agronomy. 2022; 12(4):808. https://doi.org/10.3390/agronomy12040808

Chicago/Turabian Style

Kim, JangChol, Xin An, Ke Yang, Si Miao, Yushi Qin, Yinxia Hu, Bo Du, Lili Zhu, Guangcun He, and Rongzhi Chen. 2022. "Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.)" Agronomy 12, no. 4: 808. https://doi.org/10.3390/agronomy12040808

APA Style

Kim, J., An, X., Yang, K., Miao, S., Qin, Y., Hu, Y., Du, B., Zhu, L., He, G., & Chen, R. (2022). Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.). Agronomy, 12(4), 808. https://doi.org/10.3390/agronomy12040808

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Molecular Mapping of a New Brown Planthopper Resistance Gene Bph43 in Rice (Oryza sativa L.)

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