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Agronomy
Volume 12
Issue 3
10.3390/agronomy12030694
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Communication

Expression of the VvMYB60 Transcription Factor Is Restricted to Guard Cells and Correlates with the Stomatal Conductance of the Grape Leaf

by
Fabio Simeoni
1,†,
Laura Simoni
1,†,
Michela Zottini
2,
Lucio Conti
1,
Chiara Tonelli
1,
Giulia Castorina
3,
Luca Espen
3 and
Massimo Galbiati
4,*
1
Department of Biosciences, Università degli Studi di Milano, 20133 Milan, Italy
2
Department of Biology, Università degli Studi di Padova, 35131 Padua, Italy
3
Department of Agricultural and Environmental Sciences—Production, Landscape, Agroenergy (DiSAA), Università degli Studi di Milano, Via Celoria 2, 20133 Milano, Italy
4
Institute of Agricultural Biology and Biotechnology, National Research Council, 20133 Milan, Italy
*
Author to whom correspondence should be addressed.
†
These authors contributed equally to this work.
Agronomy 2022, 12(3), 694; https://doi.org/10.3390/agronomy12030694
Submission received: 11 February 2022 / Revised: 3 March 2022 / Accepted: 9 March 2022 / Published: 13 March 2022
(This article belongs to the Special Issue Current Progress in Improving Water Use Efficiency of Vineyards)
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Abstract

The modulation of stomatal activity is a relevant trait in grapes, as it defines the isohydric/anysohydric behavior of different cultivars and directly affects water-use efficiency and drought resistance of vineyards. The grape transcription factor VvMYB60 has been proposed as a transcriptional regulator of stomatal responses based on its ectopic expression in heterologous systems. Here, we directly addressed the cellular specificity of VvMYB60 expression in grape leaves by integrating independent approaches, including the qPCR analysis of purified stomata and the transient expression of a VvMYB60 promoter: GFP fusion. We also investigated changes in the VvMYB60 expression in different rootstocks in response to declining water availability. Our results indicate that VvMYB60 is specifically expressed in guard cells and that its expression tightly correlates with the level of stomatal conductance (gs) of the grape leaf. As a whole, these findings highlight the relevance of the VvMYB60 regulatory network in mediating stomatal activity in grapes.
Keywords: guard cell; laser microdissection; MYB transcription factor; gene expression; stomatal conductance guard cell; laser microdissection; MYB transcription factor; gene expression; stomatal conductance

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MDPI and ACS Style

Simeoni, F.; Simoni, L.; Zottini, M.; Conti, L.; Tonelli, C.; Castorina, G.; Espen, L.; Galbiati, M. Expression of the VvMYB60 Transcription Factor Is Restricted to Guard Cells and Correlates with the Stomatal Conductance of the Grape Leaf. Agronomy 2022, 12, 694. https://doi.org/10.3390/agronomy12030694

AMA Style

Simeoni F, Simoni L, Zottini M, Conti L, Tonelli C, Castorina G, Espen L, Galbiati M. Expression of the VvMYB60 Transcription Factor Is Restricted to Guard Cells and Correlates with the Stomatal Conductance of the Grape Leaf. Agronomy. 2022; 12(3):694. https://doi.org/10.3390/agronomy12030694

Chicago/Turabian Style

Simeoni, Fabio, Laura Simoni, Michela Zottini, Lucio Conti, Chiara Tonelli, Giulia Castorina, Luca Espen, and Massimo Galbiati. 2022. "Expression of the VvMYB60 Transcription Factor Is Restricted to Guard Cells and Correlates with the Stomatal Conductance of the Grape Leaf" Agronomy 12, no. 3: 694. https://doi.org/10.3390/agronomy12030694

APA Style

Simeoni, F., Simoni, L., Zottini, M., Conti, L., Tonelli, C., Castorina, G., Espen, L., & Galbiati, M. (2022). Expression of the VvMYB60 Transcription Factor Is Restricted to Guard Cells and Correlates with the Stomatal Conductance of the Grape Leaf. Agronomy, 12(3), 694. https://doi.org/10.3390/agronomy12030694

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