Applied Selenium as a Powerful Antioxidant to Mitigate the Harmful Effects of Salinity Stress in Snap Bean Seedlings
Abstract
:1. Introduction
2. Materials and Methods
2.1. Plant Material, Growth Conditions and Treatments
2.2. Determination of Growth Parameters and Leaf Pigments
2.3. Quantification of Relative Water Content and Osmolytes
2.4. Measurements of Lipid Peroxidation and H2O2
2.5. Determination of Antioxidant Enzyme Activities
2.6. Quantification of Minerals Content
2.7. Genes Relative Expression by qRT-PCR
2.8. Statistical Analysis and Figures Preparation
3. Results
3.1. Effect of Applied-Se on Vegetative Growth
3.2. Effect of Applied-Se on Leaf Pigments
3.3. Effect of Applied Se on RWC and Osmolytes
3.4. Effect of Applied-Se on the Accumulation of MDA and H2O2
3.5. Effect of Applied Se on the Activities of Antioxidant Enzymes
3.6. Effect of Applied Se on the Mineral Contents
3.7. Effect of Applied Se on the Expression of Sulfate Transporter (SULTRs) Genes
3.8. Effect of Applied Se on the Salt Stress Responsive Genes
4. Discussion
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Sequence | |
---|---|---|
SULTR1 | F | 5′-CGCAGACTATGAATACCCGA-3′ |
R | 5′-TTCCTAAACGGGTCATCTGG-3′ | |
SULTR2 | F | 5′-CAGAAGGAATAGCAATAGGA-3′ |
R | 5′-CAAGTAGCAGGAAGTAAAAG-3′ | |
SULTR3 | F | 5′-TCTTTCTCACGGTCAGCAGT-3′ |
R | 5′-TAGCATTTGGAGTGTATTCG-3′ | |
SOS1 | F | 5′-ACTTGCAGGAGGAATACAAC-3′ |
R | 5′-CGAGAAGAGAAGACCACATC-3′ | |
Osmotin | F | 5′-GAACGGAGGGTGTCACAAAATC-3′ |
R | 5′-CGTAGTGGGTCCACAAGTTCCT-3′ | |
NHX1 | F | 5′-CGTGATGTCGCATTACACCT-3′ |
R | 5′-CTGGCAAACTCCCACTTCTC-3′ | |
GAPDH | F | 5′-TGACGACATCAAGAAGGTGGTG-3′ |
R | 5′-GAAGGTGGAGGAGTGGGTGTC-3′ |
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Farag, H.A.S.; Ibrahim, M.F.M.; El-Yazied, A.A.; El-Beltagi, H.S.; El-Gawad, H.G.A.; Alqurashi, M.; Shalaby, T.A.; Mansour, A.T.; Alkhateeb, A.A.; Farag, R. Applied Selenium as a Powerful Antioxidant to Mitigate the Harmful Effects of Salinity Stress in Snap Bean Seedlings. Agronomy 2022, 12, 3215. https://doi.org/10.3390/agronomy12123215
Farag HAS, Ibrahim MFM, El-Yazied AA, El-Beltagi HS, El-Gawad HGA, Alqurashi M, Shalaby TA, Mansour AT, Alkhateeb AA, Farag R. Applied Selenium as a Powerful Antioxidant to Mitigate the Harmful Effects of Salinity Stress in Snap Bean Seedlings. Agronomy. 2022; 12(12):3215. https://doi.org/10.3390/agronomy12123215
Chicago/Turabian StyleFarag, Hoda A. S., Mohamed F. M. Ibrahim, Ahmed Abou El-Yazied, Hossam S. El-Beltagi, Hany G. Abd El-Gawad, Mohammed Alqurashi, Tarek A. Shalaby, Abdallah Tageldein Mansour, Abdulmalik A. Alkhateeb, and Reham Farag. 2022. "Applied Selenium as a Powerful Antioxidant to Mitigate the Harmful Effects of Salinity Stress in Snap Bean Seedlings" Agronomy 12, no. 12: 3215. https://doi.org/10.3390/agronomy12123215