Agronomic Performance of RR® Soybean Submitted to Glyphosate Application Associated with a Product Based on Bacillus subtilis
, Eduardo Souza Freire 1, Bruno César Silva Pereira 1, Fernanda dos Santos Farnese 2, Matheus de Freitas Souza 1, Lucas Loram-Lourenço 2 and Letícia Ferreira de Sousa 2
Round 1
Reviewer 1 Report (Previous Reviewer 4)
I feel authors could to significant changes in the manuscript to improve its quality. But I do not see any major changes in the revised version.
Author Response
Please see the attachment.
Author Response File: 
Author Response.pdf
Reviewer 2 Report (Previous Reviewer 3)
Corrections are done.
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 3 Report (Previous Reviewer 2)
The authors made all suggested changes, looks good
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Reviewer 4 Report (Previous Reviewer 1)
The introduction provides sufficient background and includes all relevant references to investigate the use of growth-promoting microorganisms to attenuate injuries caused by herbicides. In this case, the agronomic performance of RR® soybean submitted to post-emergence application of glyphosate isolated and in association with Bacillus subtilis. All the cited references are relevant to the research. The research design Is appropriate. Methods are adequately described. The results are clearly presented. The results support the conclusions.
Line 399: PSII instead of ΦPSII
Line 401: Medicago instead of Mendicago
Author Response
Please see the attachment.
Author Response File: Author Response.pdf
Round 2
Reviewer 1 Report (Previous Reviewer 4)
Manuscript looks fine.
This manuscript is a resubmission of an earlier submission. The following is a list of the peer review reports and author responses from that submission.
Round 1
Reviewer 1 Report
The introduction provides sufficient background and includes all relevant references to investigate the use of growth-promoting microorganisms to attenuate injuries caused by herbicides and in this case the agronomic performance of RR® soybean submitted to post-emergence application of glyphosate isolated and in association with Bacillus subtilis. All the cited references are relevant to the research. The research design Is appropriate. Methods are adequately described. The results are clearly presented. The conclusions are supported by the results.
The key is to see if this effect is also be seen in weeds, which is rather unwelcome.
Line 305-308: why don't we see the same EL value in the GLY D2 treatment?
Reviewer 2 Report
Expand RR when it appears first
Line nos. 15-17 Please clearly mention the doses; Using 2x for multiple applications is confusing as the weed science community uses x for depicting times of field dose.
Line o. 19 "this herbicide"? Which herbicide?
Line 21-22 Not clear; please rewrite
Line 63-64 Are the microorganisms used as plant growth regulators or biocontrol agents? Both are different. Please clarify.
Line 69-64 Not sure how robust this hypothesis is; please include more references
Line 83 Please provide the soil type in English.
Reviewer 3 Report
Only:
line 80: carry out instead installed
line: 398: B. amyloliquefaciens - in italics
Why 1 year field study only?
Reviewer 4 Report
1. Introduction must include and discuss previous studies.
2. It is unclear why authors select Bacillus subtilis for this study. There are several biocontrol agents present.
3. The study lacks scientific motivation and novelty.
4. Authors do not demonstrate mechanistic aspects of the Bacillus subtilis in intoxication.
5. Authors need more biological experiments and data to support their claim. Visual scale and chlorophyll content is not enough to make such a claim.
6. Visual examination and chlorophyll levels can vary due to several other reasons.
7. Figures quality need to be improved.
