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Barley Plants Overexpressing Ferrochelatases (HvFC1 and HvFC2) Show Improved Photosynthetic Rates and Have Reduced Photo-Oxidative Damage under Drought Stress than Non-Transgenic Controls
Article

Altering Tetrapyrrole Biosynthesis by Overexpressing Ferrochelatases (Fc1 and Fc2) Improves Photosynthetic Efficiency in Transgenic Barley

1
School of Agriculture, Food and Wine, University of Adelaide, Waite Campus, PMB1, Glen Osmond, SA 5064, Australia
2
CSIRO Agriculture & Food, Locked Bag 2, Glen Osmond, SA 5064, Australia
3
Wheat Initiative, Julius-Kühn-Institute, Königin-Luise-Str 19, 14195 Berlin, Germany
*
Author to whom correspondence should be addressed.
Agronomy 2020, 10(9), 1370; https://doi.org/10.3390/agronomy10091370
Received: 14 August 2020 / Revised: 7 September 2020 / Accepted: 7 September 2020 / Published: 11 September 2020
Ferrochelatase (FC) is the terminal enzyme of heme biosynthesis. In photosynthetic organisms studied so far, there is evidence for two FC isoforms, which are encoded by two genes (FC1 and FC2). Previous studies suggest that these two genes are required for the production of two physiologically distinct heme pools with only FC2-derived heme involved in photosynthesis. We characterised two FCs in barley (Hordeum vulgare L.). The two HvFC isoforms share a common catalytic domain, but HvFC2 additionally contains a C-terminal chlorophyll a/b binding (CAB) domain. Both HvFCs are highly expressed in photosynthetic tissues, with HvFC1 transcripts also being abundant in non-photosynthetic tissues. To determine whether these isoforms differentially affect photosynthesis, transgenic barley ectopically overexpressing HvFC1 and HvFC2 were generated and evaluated for photosynthetic performance. In each case, transgenics exhibited improved photosynthetic rate (Asat), stomatal conductance (gs) and carboxylation efficiency (CE), showing that both FC1 and FC2 play important roles in photosynthesis. Our finding that modified FC expression can improve photosynthesis up to ~13% under controlled growth conditions now requires further research to determine if this can be translated to improved yield performance under field conditions. View Full-Text
Keywords: tetrapyrrole; ferrochelatase; heme; chlorophyll; photosynthesis tetrapyrrole; ferrochelatase; heme; chlorophyll; photosynthesis
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MDPI and ACS Style

Nagahatenna, D.S.K.; Tiong, J.; Edwards, E.J.; Langridge, P.; Whitford, R. Altering Tetrapyrrole Biosynthesis by Overexpressing Ferrochelatases (Fc1 and Fc2) Improves Photosynthetic Efficiency in Transgenic Barley. Agronomy 2020, 10, 1370. https://doi.org/10.3390/agronomy10091370

AMA Style

Nagahatenna DSK, Tiong J, Edwards EJ, Langridge P, Whitford R. Altering Tetrapyrrole Biosynthesis by Overexpressing Ferrochelatases (Fc1 and Fc2) Improves Photosynthetic Efficiency in Transgenic Barley. Agronomy. 2020; 10(9):1370. https://doi.org/10.3390/agronomy10091370

Chicago/Turabian Style

Nagahatenna, Dilrukshi S.K., Jingwen Tiong, Everard J. Edwards, Peter Langridge, and Ryan Whitford. 2020. "Altering Tetrapyrrole Biosynthesis by Overexpressing Ferrochelatases (Fc1 and Fc2) Improves Photosynthetic Efficiency in Transgenic Barley" Agronomy 10, no. 9: 1370. https://doi.org/10.3390/agronomy10091370

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