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Article

22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm

National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
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Author to whom correspondence should be addressed.
These authors have contributed equally to this work.
Agronomy 2020, 10(5), 624; https://doi.org/10.3390/agronomy10050624
Received: 1 April 2020 / Revised: 16 April 2020 / Accepted: 21 April 2020 / Published: 28 April 2020
(This article belongs to the Special Issue Maize Breeding for Alternative and Multiple Uses)
Starch, the main form of stored energy in plants, plays an important role in maize (Zea mays L.) kernel development. The Shrunken-2 (Sh2) gene encodes the large subunit of the rate-limiting starch biosynthetic enzyme ADP-glucose pyrophosphorylase (AGPase). The sh2 mutant exhibits impaired AGPase activity, resulting in the partial or complete loss of starch synthesis. Here, we investigated the transcriptional regulatory framework of sh2 through transcriptome and co-expression network analysis using an F2 population derived from the maize reference line B73 and sweet corn inbred line HZ508. We identified 5175 differentially expressed genes (DEGs), including 2878 upregulated and 2297 downregulated genes in sh2 mutant lines. DEGs are associated with various biological processes including nutrient reservoir activity, transferase activity, catalytic activity, water deprivation and glycogen metabolism. At the genetic level, 2465 DEGs, including 357 transcription factors, were involved in transcription. In addition, the maize floury and opaque mutant genes fl1, ndk2, o7 and o2, which regulate the biosynthesis of 22KD zein, were co-expressed with the differential expressed transcription factor genes, thus suggesting that zein content might be a key regulator coordinating the expression of genes determining starch accumulation in maize endosperm. View Full-Text
Keywords: endosperm development; maize; sh2; starch accumulation; zein endosperm development; maize; sh2; starch accumulation; zein
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MDPI and ACS Style

Nelly Sandrine, A.M.; Zhao, H.; Qin, Y.; Sun, Q.; Gong, D.; Pan, Z.; Qiu, F. 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm. Agronomy 2020, 10, 624. https://doi.org/10.3390/agronomy10050624

AMA Style

Nelly Sandrine AM, Zhao H, Qin Y, Sun Q, Gong D, Pan Z, Qiu F. 22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm. Agronomy. 2020; 10(5):624. https://doi.org/10.3390/agronomy10050624

Chicago/Turabian Style

Nelly Sandrine, Ada M., Hailiang Zhao, Yao Qin, Qin Sun, Dianming Gong, Zhenyuan Pan, and Fazhan Qiu. 2020. "22KD Zein Content Coordinates Transcriptional Activity during Starch Synthesis in Maize Endosperm" Agronomy 10, no. 5: 624. https://doi.org/10.3390/agronomy10050624

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