Assessment of 16 Peanut (Arachis hypogaea L.) CSSLs Derived from an Interspecific Cross for Yield and Yield Component Traits: QTL Validation
Centre d’Etude Régional pour l’Amélioration de l’Adaptation à la Sécheresse (CERAAS), Institut Sénégalais de Recherches Agricoles (ISRA), Route de Khombole, Thiès BP 3320, Senegal
Département de Biologie Végétale, Faculté des Sciences et Techniques, Université Cheih Anta Diop de Dakar, Dakar BP 5005, Senegal
Institut Universitaire de Technologies Bois-Mbalmayo, Université de Yaoundé 1, Mbalmayo BP 306, Yaoundé, Cameroun
Centre National de Recherche Agronomique (CNRA), Institut Sénégalais de Recherches Agricoles (ISRA), Bambey BP 53, Senegal
CIRAD, UMR AGAP, F-34398 Montpellier, France
AGAP, Univ Montpellier, CIRAD, INRAE, Montpellier SupAgro, 34090 Montpellier, France
CIRAD, UMR AGAP, Thies BP 3320, Senegal
Author to whom correspondence should be addressed.
Agronomy 2020, 10(4), 583; https://doi.org/10.3390/agronomy10040583
Received: 8 April 2020 / Revised: 14 April 2020 / Accepted: 15 April 2020 / Published: 18 April 2020
(This article belongs to the Section Crop Breeding and Genetics)
Cultivated peanut is an allotetraploid (2n = 4× = 40) with narrow genetic diversity. In previous studies, we developed an advanced backcross quantitative trait loci (AB-QTL) population from the cross between the synthetic allotetraploid ((Arachis ipaensis × Arachis duranensis)4×) and the cultivated variety Fleur11, and mapped several quantitative trait loci (QTLs) involved in yield and yield components. We also developed a chromosome segment substitution line (CSSL) population as a way to mendelize the QTLs and analyzing their effects. In this study, 16 CSSLs were used for assessing the contribution of wild alleles in yield performance and stability across environments, as well as validating QTLs for pod and seed size. The CSSLs and the recurrent parent Fleur11, used as a check, were assessed using an alpha lattice design in three locations during two consecutive rainy seasons in Senegal, totaling six environments. Our results showed that the chromosome segments from the wild species, in general, have no yield disadvantage and contributed positive variation to yield-related traits. Most of the QTLs detected for pod and seed size in the AB-QTL on linkage groups A07, A08, A09, and B06 were also found in the CSSLs, showing that the CSSLs used in this study are accurate material for QTL validation. Several new QTLs have also been identified. Two CSSLs (12CS_031 and 12CS_069) showed consistently higher pod and seed size than Fleur11 in all environments, suggesting that the QTLs were consistent and stable. Our study opens the way for pyramiding these QTLs for peanut improvement.